Figure 1.
Time points of the transcriptome experiments.
A and B are duplicate control samples; D and E are duplicate samples treated with fusaricidin after the 7-h culture of B. subtilis 168.
Figure 2.
Protein-protein interaction networks at 5 min using the string analysis.
Figure 3.
The rapid-response pathways of B. subtilis to the fusaricidin treatment.
Fus, fusaricidin. The red columns indicate the hypothetical proteins translated from the genes in the corresponding blue ellipses.
Figure 4.
The metabolic changes of carbon and nitrogen.
The expression of genes related to the central carbon and nitrogen pathways are schematically presented. The 3 bars from left to right represent the fold changes of the gene expressions in response to the 3 time points (5, 20, and 170 min). The red bars represent an upregulation; the green bars, a downregulation; and the gray bars, the messages that did not significantly change relative to our cutoff (3-fold increase in expression).
Table 1.
The MIPS analysis of the differential genes at 20 min.
Figure 5.
Changes in nucleotide metabolism.
The expression of genes related to nucleotide metabolism are schematically presented. The 3 bars from left to right represent the fold changes of the gene expressions in response to the 3 time points (5, 20, and 170 min). The red bars represent an upregulation; the green bars, a downregulation; and the gray bars, the messages that did not significantly change relative to our cutoff (3-fold increase in expression).
Table 2.
Gene groups with E <0.05 at 5, 20, and 170 min.
Figure 6.
The 3 bars from left to right represent the fold changes of the gene expressions in response to the 3 time points (5, 20, and 170 min). The red bars represent an upregulation; the green bars, a downregulation; and the gray bars, the messages that did not significantly change relative to our cutoff (3-fold increase in expression).
Figure 7.
The transport and oxidation stress response associated with Fe2+ and Mn2+. Fus, fusaricidin.
Figure 8.
Clustering analysis of 6 experiments.
Six individual experiments are listed on the top of the figure, and the names of the genes are shown on the right. The similarities of the genes between the different experiments are indicated in different colors. Low expression is indicated in green; and high expression, in red.
Figure 9.
The clustering analysis between the antibiotic microarray data.
Different antibiotics are listed on the top of the figure. The similarities of the genes between the different experiments are indicated in different colors. Low expression is indicated in green; and high expression, in red.