Table 1.
Strains included in either the MAT1-1 or MAT1-2 alignment for analyzing selection pressures.
Figure 1.
Schematic illustration of the Mating-type locus and the flanking of Penicillium roqueforti FM 164 (MAT1-2 strain) and its close relative P. chrysogenum Wisconsin 54–1255 (MAT1-1 strain).
The transcriptional direction of the genes is indicated by an arrow. As the Wisconsin strain is a MAT1-1 strain, we identified the MAT1-2 gene in another P. chrysogenum strain (AM904545). Genes represented in both species by same colors show a nucleotide identity>80%.
Figure 2.
Color coded Selecton Results for a) MAT1-1 and b) MAT1-2 sequences of Penicillium roqueforti.
Amino acids sites under purifying selection are colored in purple. Darker purple colors indicate stronger purifying selection and values of ω closer to 0. More than 70% of the sites for MAT1-1 and 50% for MAT1-2 are evolving under strong purifying selection.
Figure 3.
RIP mutation frequency plot over a rolling sequence window, corresponding to the multiple alignment (not shown).
Nucleotide polymorphisms (against the alignment consensus, which is also the highest GC-content sequence) mostly correspond to CpA<- ->TpA or TpG<- ->TpA (red curve), as expected when RIP is acting.