Table 1.
Body weight loss (g) in Ve or FSG-treated colitic rats.
Figure 1.
Effects of FSG treatment on the severity of TNBS-induced colitis.
(A) MDS, (B) MD, (C) MPO activity were evaluated in presence or absence of the ER antagonist ICI 182.780. Values are presented as means ± SEM. Statistical analysis were performed by One way-ANOVA with post hoc comparison by Tukey test. *p<0.05 vs noninflamed group, **p<0.01 vs noninflamed group, #p<0.05 vs inflamed vehicle, ##p<0.01 vs inflamed vehicle, ap<0.05 vs Ve-treated rats 3 d post-TNBS, bp<0.05 vs Ve-treated rats 24 h and 5 d post-TNBS.
Figure 2.
Effects of FSG treatment on cytokine profile after TNBS colitis.
Effects of FSG on (A) MIF expression, (B) IL-1β, (C) IL-10 levels were evaluated in non inflamed colon and 24 h, 3 d, 5 d after TNBS-induced colitis in presence or absence of the ER antagonist ICI 182.780. Values are presented as means ± SEM. Statistical analysis were performed by One way-ANOVA with post hoc comparison by Tukey test. *p<0.05 vs non inflamed group, **p<0.01 vs non inflamed group, #p<0.05 vs inflamed vehicle, ##p<0.01 vs inflamed vehicle, ap<0.05 vs Ve-treated rats 24 h post-TNBS, bp<0.05 vs Ve-treated rats 3 d post-TNBS.
Figure 3.
Effects of FSG treatment on TNBS-induced changes in intestinal paracellular permeability ± ER antagonist ICI182.780.
Values are presented as means ± SEM. Statistical analysis were performed by One way-ANOVA with post hoc comparison by Tukey test. *p<0.05 vs non inflamed group, **p<0.01 vs non inflamed group, ##p<0.01 vs inflamed vehicle, ap<0.05 vs Ve-treated rats 3 d post-TNBS.
Figure 4.
Effects of FSG treatment on (A) TNBS-induced visceral hypersensitivity in response CRD (B) colonic compliance.
Abdominal response and colonic compliance were determined on 5th day post-TNBS in presence or absence of the ER antagonist ICI 182.780. Values are presented as means ± SEM. Statistical analysis were performed by One way-ANOVA with post hoc comparison by Tukey test. *p<0.05 vs non inflamed group, **p<0.01 vs non inflamed group, #p<0.05 vs inflamed vehicle, ##p<0.01 vs inflamed vehicle.
Figure 5.
Effects of FSG treatment on TNBS-induced changes in faecal proteolytic activity ± ER antagonist ICI182.780.
Data are expressed as means ± SEM. One way-ANOVA with post hoc comparison by Tukey test was used to analyse the data. *p<0.05 vs non inflamed group, #p<0.01 vs inflamed vehicle.
Figure 6.
Immunohistochemical staining of longitudinal colonic section for PAR-2 expression.
PAR-2 expression was evaluated in colonic epithelial cells in non inflamed colon and 24 h, 3 d, 5 d after TNBS-induced colitis in vehicle (Ve) or FSG treated rats.