Table 1.
Probes used, target microorganisms and DNA target regions. [Cyanine dye (CY3); Carboxyfluorescein (FAM)].
Figure 1.
DAPI staining (A, B, C); Pan-Flavo probe (D, E, F); F. psychrophilum probes (G, H, I) (100x). F. psychrophilum (DSM3660) (A, D, G); Flavobacterium spp. (B, E, H); Chryseobacterium spp. (C, F, I).
Table 2.
Agreement between FISH and 16S rDNA sequencing (SEQ, used as gold standard) in the experiments carried out with the Pan-Flavo (Flavo285) probe and the combination of two F. psychrophilum (FlavoP77, FlavoP477) probes.
Figure 2.
ROC curves for cell suspension of pure strains; area under the curve (AUC) for FISH: 0.89, for culture method: 0.79.
(A). ROC curves for spiked spleens; AUC for FISH: 0.84, for culture method: 0.6 (B).
Figure 3.
FISH assay on infected fish tissues.
Pan-Flavo probe (A, B); F. psychrophilum probes (C, D). F. psychrophilum on skin (A, C) and F. psychrophilum in a spleen (B, D).