Table 1.
Differentially expressed probe sets in SoS in basal situation.
Table 2.
Differentially expressed probe sets in SoS after stimulation with RA.
Table 3.
Signaling pathways analysis results.
Figure 1.
Geneplots of the probe sets influencing the MAPK pathway.
Geneplots are shown for the 50 most influential probe sets from the KEGG MAPK pathway (A) and for the GO-term MAPKKK cascade (B) after stimulation with RA that contribute to the differential pathway expression in SoS and control. Probe sets are scaled to unit standard deviations and the height of the bars are the number of standard deviations above the cut-off level of 0.7. Higher bars indicate higher influence on the pathway. Probe sets with the highest influence on the pathway (i.e. FGF13 in Figure 1A and TNIK in Figure 1B) are depicted on the left. Corresponding gene names are written below each bar.
Figure 2.
NSD1 knock down increases RASIP1 expression.
Expression of NSD1 was downregulated with siRNA (black bar on the left), which resulted after 48-hours in up regulation of RASIP1 expression (black bar on the right), while no difference in expression was detected for the controls (white and grey bars). Fold change represents the average difference in expression level of the respective gene. Fold changes were adjusted for the expression of the housekeeping gene β2 microglobulin using the 2−ΔΔCt method.
Figure 3.
bFGF induced SBE reporter activation is potentiated by RASIP1.
Values are expressed as fold induction compared to control. Cells were stimulated with bFGF for 24 hours. Control was not stimulated with bFGF and no RASIP1 was co-transfected. Co-tranfection of RASIP1 did not affect basal reporter activity. bFGF (10 ng/ml) significantly stimulated SBE reporter activity (indicated with #; p<0.05). RASIP1 enhanced bFGF induced reporter dose-dependently (indicated with *; p<0.05).
Figure 4.
The results are shown for the phosphorylation levels for the MEK1 (A), ERK1/ERK2 (B), ERK2 (C), p38MAPK (D), cJUN (E), ATF2 (F), JNK (G), HSP27 (H) and p90RSK kinases (I). Bar heights depict the mean fluorescence intensity levels measured (MFI) and the p-values for the difference between SoS and control (after correction for total protein levels) are shown above the bars.