Figure 1.
3D-OCT image acquisition of the co-cultures, and analysis of the shape and size of acini.
A. 3D-OCT image acquistion: the surface of the gel is aligned near the top of each image, and the depth-resolved light scattering from cells beneath the gel surface is apparent at depths up to ∼1 mm; segmentation of acini to characterize the overall size and the lumen is also shown. B. Temporal changes in acini and lumen sizes analyzed from 3D-OCT images of the co-cultures. C. An example isosurface rendering of an acinus from a 3D-OCT image-stack; slicing of the rendered volume clearly shows the lumen. D. An example 3D rendering of an aspherical acinus.
Figure 2.
Comparison of MCF10A:RMF co-cultures with MCF10DCIS.com:RMF co-cultures shows significantly larger acini and lumen sizes (Student’s t-test, p-value <0.005) at week 2. In MCF10DCIS.com:RMF co-cultures, acini and lumen size are also observed to be highly modulated by the ratio of fibroblasts.
Figure 3.
The minimum asphericity value of 1 indicates a perfect sphere, while less spherical acini have higher asphericity values. Acini comprised of MCF10DCIS.com cells are seen to become increasingly aspherical in the presence of fibroblasts.