Figure 1.
Kinetics of OPN Full and N-half in synovial fluid after joint injury.
(Left panels) Time course changes of OPN Full (upper panel) and OPN N-half (lower panel) protein levels in synovial fluid after ACL rupture. Open bar: within 1 month after rupture n = 14, Closed bar: greater than 1 month after rupture n = 68. (Right panels) OPN Full (upper panel) and OPN N-half (lower panel) protein levels in synovial fluid collected from pre (n = 23) and post (n = 93) ACL reconstruction surgery. Data are indicated mean+/− SEM. **; p<0.01.
Figure 2.
OPN Full levels in synovial fluid are positively correlated with joint damage in lateral tibial plateau.
Severity of articular cartilage damage was scored according to the protocol described by Asano et al [32] with minor modification (Table S1). Open bar; intact articular cartilage (score = 1) Closed bar; damaged articular cartilage (score = 2∼6). Number of samples is indicated below each column. Data are indicated mean+/− SEM. *; p<0.05.
Figure 3.
OPN Full and N-half levels in synovial fluid are positively correlated with the severity of joint pain within a month after ACL rupture.
VAS indicating constitutive pain in a daily life (see Table S2) was collected in the examination room before consultation. Open circle; VAS from patients within 1 month after ACL rupture. Closed circle; VAS from patients who ruptured ACL greater than 1 month ago. Number of samples, correlation coefficient, and p value are indicated in each figure.
Figure 4.
Correlation of OPN Full and N-half levels in synovial fluid with Lysholm scores.
Lysholm scores (see Table S3) were collected in an examination room by expert joint surgeons in our university hospital. The basis of the classification of Lysholm score was 25:feel no pain at any time, 20:feel not so severe or tolerable pain even after severe exertion, 0–15:feel severe or intolerable pain sometimes in a daily life. Numbers of samples are indicated below each figure.
Figure 5.
OPN N-half levels in synovial fluid are negatively correlated with the severity of joint pain in patients who ruptured ACL greater than 1 month ago.
VAS was collected from patients who ruptured ACL more than 1 month ago using a questionnaire described in Table S2 in an examination room at our university hospital. Number of samples, correlation coefficient, and p value are indicated in each figure.
Figure 6.
OPN Full and N-half levels in synovial fluid are independent from systemic inflammatory status but associate with joint inflammation.
(Left and Middle panels) Blood samples were collected pre- and post-operation (at day 4) and serum CRP levels were quantified at the clinical laboratory of our university hospital. (Right panels) Synovial fluid was collected just before operation in a surgery room. Number of samples, correlation coefficient, and p value are indicated in each figure.