Figure 1.
Histological analysis showing the types of nasal polyps in Chinese patients: eosinophilic nasal polyps.
A). Nasal endoscopic findings; B). Nasal endoscopic findings; C). Histological appearance at 200×magnification; D). Histological appearance at 400×magnification.
Table 1.
The clinical characteristics of the patients with nasal polyps for SAPAS and q-PCR analysis.
Table 2.
Summary statistics of the SAPAS data from Illumina GA IIx sequencing.
Figure 2.
The characteristics of the SAPAS data.
A) The distribution of numbers of poly(A) sites per gene. B) The distribution of the number of reads per UCSC canonical gene. C) The genomic locations of the reads that were uniquely mapped to the nuclear genome after internal priming filtering. D) The genomic locations of the poly(A) sites in all genes.
Figure 3.
An analysis of APA switching genes.
A). APA site switching and gene expression levels of nasal mucosa and nasal polyp. The x-axis denotes the r-value. A larger positive value shows that nasal polyp samples are prone to use longer tandem 3′UTRs. Genes with significant switching to longer (blue) or shorter (red) tandem UTRs in nasal polyp samples (FDR = 0.01; see Methods) are colored. The y-axis denotes the logarithm of the expression levels of genes from the nasal polyp sample corresponding to the nasal mucosa sample. B). Experimentally validated mir-377 and mir-146a target sites in 3′UTR of SOD1 and STAT1 mRNA.
Figure 4.
Venn diagram of genes with 3′UTR length changes between the two cases.
Table 3.
Enrichment of genes with significantly shorter or longer 3′UTR isoforms involved in various GO functional categories.
Table 4.
Twelve genes enriched in GO terms associated with apoptosis.
Table 5.
Eighteen genes enriched in GO terms associated with transcription.
Table 6.
Four genes enriched in the wnt pathway.
Figure 5.
Real-Time PCR analysis in 10 additional patients.
A) BCAP29. B) SOD1. C) DEDD. D) TAX1BP. E) c2orf68. F) Ube2e2. G) C8orf84. cUTR/eUTR: constitutive UTR/extended UTR, the expression ratios of the shortened region to the lengthened region. *: p<0.05.