Table 1.
Patients characteristics according to HF aetiology.
Figure 1.
Influence of heart failure aetiology on the levels of nucleoporins.
NDC1, Nup155, Nup160, Nup153, Nup93 and TPR were analysed by Western blot techniques. The values from the CNT group were set to 100. The data are expressed as mean ± SEM in arbitrary units (optical density) of six independent experiments. ICM, ischaemic cardiomyopathy; DCM, dilated cardiomyopathy; CNT, control. **p<0.01, ***p<0.0001 vs. CNT group. ##p<0.01, ICM vs. DCM group.
Table 2.
Results of the expression of nuclear proteins in HF patients and controls.
Figure 2.
Relationships between Nup160 and NDC1 levels.
Subjects with ICM (A), DCM (B) and all patients (C). Values are normalized to β-actin and finally to CNT group.
Figure 3.
Relationships between Nup160 levels and ventricular function parameters.
A) LVEDD (left ventricular end-diastolic diameter), B) LVESD (left ventricular end-systolic diameter) in HF patients group (ICM and DCM). Values are normalized to β-actin and finally to CNT group.
Figure 4.
Effect of heart failure on cell distribution of some nucleoporins in left ventricular human cardiomyocytes.
Inmunofluorescence staining with and without DAPI of NDC1, Nup160 and Nup93 according to heart failure aetiology, control (CNT) (Figure A,C and E, fluorescence; Figure B, D and F, DAPI A–F), ischaemic (ICM) (Figure G, I and K, fluorescence; Figure H, J and L, DAPI G–L) and dilated (DCM) (Figure M, O and Q, fluorescence; Figure N, P and R, DAPI M–R) groups. Arrows shows the immunofluorescence due to lipofuscin particles. Scale bar = 10 µm.
Figure 5.
Immunolocalization of NDC1 in human cardiomyocytes and western blot of nucleoporins in nuclear and cytosolic fraction.
(A) Electron micrograph, in all case, gold particles (10 nm) are over nuclear pore complex; in control (a), dilated (b) and ischaemic tissue (c). The labeling is increased in ischaemics. No labely of NDC1 was observed in other nuclear structure. Scale bar = 50 nm. (B) Western blot analysis of NDC1, Nup160 and Nup93 in nuclear (Nu) and cytosolic (Cy) fraction in controls and HF patients (ICM and DCM).