Table 1.
Summary of small RNA sequencing data.
Figure 1.
Differentially expressed miRNAs in response to N deficiency.
The significantly differentially expressed miRNAs with greater than 3-fold relative change were shown.
Figure 2.
Differential expression of different miR169 species.
(A) Four different mature miR169 species. (B) Expression of different miR169 members in response to different nutrient deficiencies. Gene expression values shown are relative to the expression in plants grown under normal MS medium, for which the value is set to 1. Error bars indicate ± SE obtained from three biological repeats. Values marked by an asterisk are significantly different from the corresponding control value with Student's t-test (p<0.01; n = 3).
Table 2.
N-starvation-responsive miRNAs and their targets.
Figure 3.
Expression of targets of different nutrient responsive miRNAs under N starvation conditions.
Gene expression values shown are relative to the expression in plants grown under normal MS medium, for which the value is set to 1. Error bars indicate ± SE obtained from three biological repeats. Values marked by an asterisk are significantly different from the corresponding control value with Student's t-test (p<0.01; n = 3).
Figure 4.
miR826 is a N-starvation-induced miRNA.
(A)Comparative analysis of miR826 precursor and its target AOP2 sequences. The sequence in pink indicates mature miR826 sequence. (B) The position of miR826, AOP1, AOP2, and AOP3 genes in chromosome. (C) The cleavage sites of AOP2 transcripts. (D) Expression of miR826 and AOP2 in response to N starvation. Gene expression values shown are relative to the expression in plants grown under normal MS medium, for which the value is set to 1. Error bars indicate ± SE obtained from three biological repeats. Values marked by an asterisk are significantly different from the corresponding control value with Student's t-test (p<0.01; n = 3).
Figure 5.
miR160, miR167, and miR171 are involved in development of root system under N starvation coditions.
(A) More lateral roots in 35S::miR160a plants than WT (wild-type) plants. (B) Expression of miR160, miR167, miR171, and their targets under N starvation conditions. Gene expression values shown are relative to the expression in plants grown under normal MS medium, for which the value is set to 1. Error bars indicate ± SE obtained from three biological repeats. Values marked by an asterisk are significantly different from the corresponding control value with Student's t-test (p<0.01; n = 3). (C) A putative work model for miRNA-mediated root growth under N starvation conditions.
Table 3.
Novel miRNAs identified from deep sequencing data.
Figure 6.
Identified targets of novel miRNAs from Arabidopsis degradome data.
Vertical arrows indicate the target cleavage positions. The number indicates the number of corresponding cleavage products.