Figure 1.
NTPDase2 immunostaining does not co-localize with α-SMA in mice bladder smooth muscle.
Cryosections of mouse bladders were labeled with antibodies to NTPDase2 (A. green), α-SMA (B. red) and Topro-3 to label nuclei (B. blue). Color merged panels are shown on the right (C). White arrows indicate distinct NTPDase2 staining next to bladder smooth muscle cells. White scale bars = 10 µm.
Figure 2.
NTPDase2 immunostaining co-localize with c-kit in mice bladder.
Cryosections of mouse bladders were labeled with antibodies to NTPDase2 (B. red), c-kit (A. green) and Topro-3 to label nuclei (B. blue). Color merged panels are shown on the right (C). Merged signals of NTPDase2 and c-kit are shown as yellow (C). White arrows indicate representative NTPDase2/c-kit co-localization. White scale bars = 10 µm.
Figure 3.
NTPDase2 co-localizes with CD34 but not tryptase in mouse bladder.
Cryosections of mouse bladders were labeled with antibodies to CD34 (A, D. green), NTPDase2 (B. red), tryptase (E. red), c-kit (G. green), and Topro-3 to label nuclei (B. E. H. blue). Color merged panels are shown on the right (C, F, I). Merged signals are shown as yellow (C. F. I). White arrows indicate representative NTPDase2/CD34 co-localization (C) or CD34/tryptase co-localization (F); White asterisks indicate CD34 positive cells with no NTPDase2 staining (C). White scale bars = 10 µm.
Figure 4.
Ano1 co-localizes with CD34 in mouse bladder.
Cryosections of mouse bladders (methanol fixation) were labeled with antibodies to Ano1 (A. D. green), Cd34 (E. red) and Topro-3 to label nuclei (B. E. blue). Color merged panels are shown on the right (C. F). Merged signals of Ano1 and CD34 are shown as yellow (F). White arrows indicate representative Ano1/CD34 co-localization. White scale bars = 10 µm.
Figure 5.
NTPDase2 immunostaining co-localize with connexin 43 in mice bladder.
Cryosections of mouse bladders were labeled with antibodies to connexin 43 (A. green), NTPDase2 (B. red) and Topro-3 to label nuclei (B. blue). Color merged panels are shown on the right (C). Merged signals of NTPDase2 and connexin 43 are shown as yellow (C). White arrows indicate representative NTPDase2/connexin 43 co-localization. White scale bars = 10 µm.
Figure 6.
NTPDase2 immunostaining co-localize with vimentin, desmin, and PDGFβ receptor in mice bladder.
Cryosections of mouse bladders were labeled with antibodies to vimentin (A. green), desmin (D. green), PDGFβ receptor (G. green), and NTPDase2 (B. E. H. red) and Topro-3 to label nuclei (B. blue). Color merged panels are shown on the right (C. F. I). Merged signals of NTPDase2 and vimentin, desmin, and PDGFβ receptor are shown as yellow (C. F. I). White arrows indicate representative co-localization. White asterisks indicate non-co-localized signal of smooth muscle (I) and fibroblasts (F. I). White scale bars = 10 µm.
Figure 7.
NTPDase2 immunostaining co-localize with merlin/NF2 in mice bladder.
Cryosections of mouse bladders were labeled with antibodies to merlin/NF2 (A. green), NTPDase2 (B. red) and Topro-3 to label nuclei (B. blue). Color merged panels are shown on the right (C). Merged signals of NTPDase2 and merlin/NF2 are shown as yellow (C). White arrows indicate representative NTPDase2/merlin co-localization. White scale bars = 10 µm.
Table 1.
Summary of expression profile of molecular markers in bladder ICC.