Figure 1.
Viral colocalization with endosomes at early infection.
Representative confocal micrographs of Vero cells infected with ASFV and immunostained for viral capsid proteins p72 and pE120R (shown in red), and in green, EE marker EEA1 (A), MVB marker CD63 (B), LE marker Rab7 (C) and LY marker Lamp1 (D) at 15 minutes postinfection (mpi). Scale bars, 10 µm. Cells were infected at a moi of 10 pfu/cell and adsorption was maintained at 4°C for 90 min. Unbound virus was then washed, cells were shifted to 37°C and infection was allowed to progress for indicated times. (E) Percentages of colocalization events of p72 capsid protein with EE or LE marker are expressed as means and relativized to the total cell-associated virus particles per individual cell at each time point in 10 cells in duplicates. (F) Percentages of colocalization events of p150 inner core protein with LE marker expressed as means and relativized to the total cell-associated virus particles per individual cell at each time point in 10 cells in duplicates. (G) Representative confocal micrograph of the colocalization of viral cores with Rab7 positive endosomes. Nuclei were stained with TOPRO3. (G1–4) Detail of colocalization between viral cores and LE in high magnification of the boxed areas in (G). (H) Colocalization of viral core protein p150 with Lamp1 marker.
Figure 2.
Low intraluminal pH and Endocytosis are required for ASFV infectivity.
(A) Inhibition of intraluminal acidification of endosomes by Baf in a time dependent manner is shown using the pH sensitive dye lysotracker red. Bar 25 µm; mpa: minutes after Baf addition. (B–E) Inhibition of intraluminal acidification of endosomes with Baf and inhibition of endocytosis with Dyn impaired virus infectivity and neither Baf nor Dyn inhibition of early infection could be recovered with acid pH medium. (B) Early viral protein p30 expression in cells pretreated with 200 nM Baf or DMSO and pulsed for 1 h with ph 5.4 medium postadsorption or maintained at pH 7.4 for 6 hpi. Western blot with specific antibodies was quantified and normalized to protein load control values. Low early viral protein expression with Baf was not recovered by acid pH medium treatment. (C) Quantification of viral protein p30 expression at 6 hpi as determined by Western blot in cells pretreated with 80 μM Dyn or DMSO and maintained in presence of medium at pH 7.4 or pulsed at pH 5.4 for 1 h post-adsorption. (D) Flow cytometry of Vero cells pretreated with Baf and infected in medium at pH 7.4 or pulsed at pH 5.4 for 1 h post-adsorption. Infected cells were then detected by FACS and data normalized to infection rates in DMSO treated cells. (E) Flow cytometry of Vero cells pretreated with Dyn and infected in medium at pH 7.4 or pulsed at pH 5.4 for 1 h. Asterisks denote sadistically significant differences (*** P<0.001). (F) Representative FACS profiles obtained during the analysis are shown.
Figure 3.
Acid pH of the late endosome is required at early stages of ASFV infection.
(A) Early viral protein p30 expression determined at 8 hpi by Western blotting with specific antibodies, quantified and normalized to protein load control values. Acid pH requirement was evidenced by the effect of lysosomotropic drug addition at any time point within the first hpi but not thereafter. (B) Representative confocal micrograph of Baf-pretreated cells fixed after 3 hpi and immunostained for major viral capsid protein p72 (red) and LE marker Rab7 (green); Bar 10 µm. Detail of colocalization between viral capsids and LEs in Baf-treated cells; Insets are magnifications of the boxed areas in the previous image, bar 1 µm. (C) Quantification of colocalization events relativized to the total number of cell-associated virions per individual cell, performed in 130 virions and expressed as means and standard deviations from two independent experiments. Asterisks denote statistically significant differences (***P<0.001).
Figure 4.
Late endosomal compartment relevance for ASFV infection.
(A) Representative FACS profiles obtained during sorter analysis of COS-7 cells transfected with GFP-Rab7-wild type (Rab7 WT) and dominant negative mutant (GFP-Rab7-DN, T22N). R3 represents transfected cells expressing GFP to be sorted. (B) Representative confocal micrographs of transfected, sorted cells after isolation, infected with ASFV at a moi of 1 for 24 hpi and immunostained for major viral capsid protein p72 (red). Percentages of transfected infected cells decreased from 43.5% in cells expressing Rab7 WT to 1.65% in cells expressing Rab7 DN. Bar 25 µm.
Figure 5.
ASFV entry depends on endosomal membrane phosphoinositides.
(A) Quantification of cell viability at 24 h by Trypan blue exclusion to determine the working concentration of PI3K inhibitor wortmannin. (B) Quantification of ASFV infectivity at 3 hpi (moi of 0.5 pfu/cell) in the presence of increasing concentrations of wortmannin. Data are expressed as percentages of infected cells from 30 random fields in triplicates and are means ± SD from three independent experiments. Asterisks denote statistically significant differences ***P<0.001. Representative confocal micrographs of cells immunostained for early viral protein p30 in red are shown in the right panels. Bar 20 µm. (C) Quantification of virus production in Vero cells untreated, treated with increasing concentrations of wortmannin from 2 h before adsorption during the whole infection cycle, or treated after 3 hpi. Cells were infected with ASFV at a moi of 0.5 pfu/cell for 24 hpi. Data are expressed as virus titers and are means ± SD from three independent experiments. Asterisks denote statistically significant differences ***P<0.001. (D) Viral protein expression at a range of post-infection times as determined by Western blot in cells to which 10 µM wortmannin was added 2 h before virus adsorption and maintained or left untreated.
Figure 6.
Phosphoinositide interconversion and related late endosome fusion events in ASFV infection.
(A) Quantification of virus production in cells untreated, treated with 1 µM PIKfyve inhibitor YM201636 or treated with DMSO. Data are expressed as virus titers and are means ± SD from three independent experiments. Asterisks denote statistically significant differences **P<0.01; *P<0.05 (B) Infected cell numbers in cells treated with PIKfyve inhibitor (YM201636) at several time points or an equivalent volume of DMSO. Data are expressed as the number of infected cells at 6 hpi (moi of 1 pfu/cell) from 20 random fields and are means ± SD from two independent experiments. Asterisks denote statistically significant differences (***P<0.001 and **P<0.01). (C) Representative confocal micrographs of infected and non-infected PIKfyve-treated cells, immunostained for Rab7 (green) and viral protein p72 (red). The characteristic phenotype of cytoplasmic vacuoles due to impaired endosome fusion was readily found in uninfected cells. Infected cells are recognized in the image as those harboring viral factories in red and lacked cytoplasmic vacuolization phenotype. Bar 10 µm.
Figure 7.
Model of ASFV infection progress through the endosomal pathway.
ASFV enters the host cell by clathrin-coated vesicles (CCVs) from clathrin-coated pits (CCPs) and clathrin molecules are recycled to the plasma membrane (PM) as the virus progresses to the endosomal pathway. First, virions gain access to EEs from PM. The EE compartment is characterized by the presence of Rab5 and EEA1. ASFV is then directed from the vacuolar domain of the EE to the acidic late compartments. Subsequently, the virions reach CD63 enriched membranes of MVBs. Under the acid intraluminal pH of these endosomes, viral capsid would be degraded and viral cores would reach LE which depends on the presence of Rab7. At this stage, viral cores could egress to the cytosol to reach their replication site at the perinuclear area. In this process, the PIs composition of the endosomal membrane seemed to be crucial. PtdIns3P is synthesized by PI3K and this process is inhibited by PI3K inhibitor wortmannin and PtdIns(3,5)P2, which is synthesized by the enzyme PIKfyve, a process blocked by the inhibitor YM201636. These PIs interconversions on the endosomal membrane are necessary for a successful infection.