Figure 1.
Electrophoresis profile of amplified products of the FRML-494 marker.
PCR amplification using the pair of primers P51 in the female (lane 1) and male (lane 2) gametophytes and the crossbred sporophytes (lanes 3–8) of Saccharina japonica (strain Rongfu) showing the FRML-494 marker (as indicated by the arrow) was only present in the female gametophytes and sporophytes. Lane M, 100 bp DNA ladder marker (Generay, Shanghai, China). Lane 0, control with H2O instead of DNA.
Figure 2.
Southern blot of the labeled FRML-494 marker.
Ethidium-bromide-stained agarose gel (A) of NotI- (lanes 1 and 3) and XbaI- (lanes 2 and 4) digested genomic DNA from the female (lanes 1 and 2) and male (lanes 3 and 4) gametophytes of S. japonica. Southern blot (B) of NotI- (lanes 1 and 3) and XbaI- (lanes 2 and 4) digested genomic DNA hybridized with the biotin-labeled FRML-494 marker. Lanes M1 and M2 show the D2000 and 1 kb DNA ladder molecular standard, respectively, (Tiangen, Beijing, China).
Figure 3.
FISH of the FRML-494 marker (red) on Saccharina japonica chromosomes counterstained with DAPI (blue).
(A) Metaphase chromosomes of the female gametophytes (n = 31); (B) hybridization signal of the FRML-494 marker (red) as indicated by the arrow on the same spread as image A; the inset is the enlarged chromosome where this marker was located; (C) an interphase nucleus of the female gametophytes with hybridization signal (red) as indicated by the arrow; (D) metaphase chromosomes of the male gametophytes (n = 31); (E) an interphase nucleus of the male gametophytes without a hybridization signal; (F) an interphase nucleus of the sporophytes with a hybridization signal (red) as indicated by the arrow; (G) metaphase chromosomes of the sporophytes (2n = 62); (H) hybridization signal (red) as indicated by the arrow present on only one metaphase chromosome of the same spread as image G; the inset is the enlarged chromosome where this marker was located; and the arrowheads in images D and G show the constriction of the chromosomes.
Table 1.
Length of chromosomes prepared from the male or female gametophytes of Saccharina japonica.