Figure 1.
A schematic overview: genomic DNA is fragmentized, end-repaired, phosphorylated and adenylated in the same reaction.
Adaptor ligation is followed by size-selection and PCR.
Figure 2.
Concordance of heterozygous SNPs (lines with dots) for 100 ng and 1 µg exome libraries of different multiplexity and a 500 ng 96-plex target capture library.
The average concordance for exome libraries was 99.4% with no significant difference between libraries. For the 96-plex experiment, the average concordance was 99.8%. Solid lines indicate the average allelic balance. Even in the 96-plex experiment, no bias in allelic balance is observed.