Figure 1.
Transmission electron microscopy image of NM-containing organelles.
Transmission electron microscopy image of a pigmented organelle from SN containing the NM pigment (white arrow) and lipid droplets (black arrow). The tissue was prepared as described in ref. 2 (scale bar 500 nm).
Table 1.
Amino acid content (µg/mg of isolated pigment) ± standard error of the mean (sem).
Table 2.
Elemental composition and C/N ratio of NM of SN, CAL and other brain areas a.
Table 3.
Theoretical percentage a of the various components in SN-NM, other-NM, and CAL-NM.
Figure 2.
IR spectra of the three NM types.
Comparison of IR spectra of SN-NM, other-NM (from PAL) and CAL-NM. Typical absorptions are indicated by asterisks (1230, 1194, 1163 cm−1) and arrows (1774, 1015 cm−1), for assignments see text.
Table 4.
Detailed assignment of the IR absorption bands of NM.
Figure 3.
Representative NMR spectra of NM, those shown are for the NM isolated from CAB: (A) 1D proton, (B) COSY, (C) 1H-13C HSQC, (D) 1H-13C HMBC, (E) DOSY.
Table 5.
Experimental 13C ppm values of NM pigments as determined by 2D 1H-13C HSQC, compared to known values of bovine liver dolichol [27].
Table 6.
Summary of COSY, HSCQ and HMBC interactions a.
Figure 4.
1D proton HR-MAS and solution NMR spectra of NM from PUT.
(A) HR-MAS water-presaturation pulse sequence with composite pulse; (B) solution spectrum with water suppression by gradient-tailored excitation. In the HR-MAS spectrum most major signals are shifted slightly upfield.
Figure 5.
1D proton HR-MAS NMR spectra of NM from PUT.
(A) HR-MAS diffusion-edited spectrum; (B) unfiltered HR-MAS spectrum; (C) HR-MAS CPMG sequence; (D) solution CPMG sequence. The vertical grey box shows the presence of two different signals around 5.0 ppm.
Figure 6.
Solid state 13C NMR spectra of NM from PUT.
(A) regions of interest in the zg spectrum; (B) high power decoupled spectrum. The intense signal at 110 ppm derives from the teflon insert, * indicates the spinning side band. The second (right hand) spinning side band (at ∼ 30 ppm) disappears beneath sample signals.
Table 7.
Results from the 13C solid state NMR spectrum.