Figure 1.
Alcian blue staing of in vitro differentiated basophils and normal blood basophils.
Panel A shows Alcian blue stained cells from day 9 of in vitro differentiated basophils originating from umbilical cord blood cultures stimulated with a continuous presence of 10 ng/ml IL-3. Panel B shows Alcian blue stained normal purified peripheral blood basophils.
Table 1.
Clones isolated from the basophil-like cell cDNA library.
Table 2.
Expression levels of lineage specific genes.
Figure 2.
Analysis of the proteome of in vitro differentiated basophils.
SDS-PAGE analysis of human in vitro differentiated basophils. Approximately 1 million cells were dissolved in SDS-sample buffer and separated on a 13% SDS PAGE gel. As a reference the same number of cells from a human embryonic kidney cell line HEK 293-EBNA was used. The positions of three protein bands that were excised from the gel and analyzed by tryptic digestion and mass spectroscopy analysis are indicated by arrows and marked with the proteins identified in these bands: eosinophil peroxidase (EPO) and major basic protein (MBP).