Table 1.
Synechocystis 6803 iSyn731 and Cyanothece 51142 iCyt773 model statistics.
Table 2.
Summary of connectivity restoration in Synechocystis 6803 iSyn731 and Cyanothece 51142 iCyt773 models.
Figure 1.
Comparison of model derived and experimentally measured [35] flux ranges for Synechocystis 6803 under the maximum biomass condition.
Basis is 100 millimole of CO2 plus H2CO3.
Table 3.
Comparison of 13C MFA flux measurements [35] vs. model-predicted flux ranges.
Figure 2.
Comparison of gene essentiality/viability data with predictions by a number of Synechocystis 6803 models.
(A) Tabulated growth (i.e., G) or non-growth (i.e., NG) predictions and experimental data. The first number denotes the number of GG, GNG, NGG and NGNG combinations whereas the second number signifies the number of experimentally observed lethal (or viable) mutants, and (B) Definition and comparison of specificity and sensitivity of all three models. Note that GG denotes both in silico and in vivo growth, NGG represents no growth in silico but in vivo growth. NGNG implies no growth for either in silico or in vivo, whereas GNG marks growth in silico but no growth in vivo.
Figure 3.
Venn diagram depicting (common and unique) reactions and metabolites between (A) iJN678 [32] and iSyn731, (B) iCce806 [25] and iCyt773, and (C) iSyn731 and iCyt773 models.
Figure 4.
Schematics that illustrate the thermodynamically infeasible cycles and subsequent resolution strategies.
(A) Cycles present in iJN678 [32], and (B) Cycles present in iCce805 [25]. Blue colored lines represent the original reaction directionality whereas green ones denote modified directionality to eliminate cycle.
Figure 5.
List of added reactions across pathways.
(A) iSyn731 compared to iJN678 [32], and (B) iCyt773 compared to iCce806 [25].
Figure 6.
Examples of pathways that differ between the two cyanobacteria.
(A) Nonfermentative alcohol production pathway highlighting the present and absent enzymes in Cyanothece 51142 and Synechocystis 6803, and (B) Alkane biosynthesis pathways in Cyanothece 51142 and Synechocystis 6803.