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Table 1.

Equations for the initial susceptible populations at seroprevalence pi.

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Figure 1.

Change in monthly mean temperatures in Singapore.

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Figure 2.

Change in the threshold mosquito density in Singapore from the 1980s to the 2000s.

Simulation 1: Effect of only temperature change with single serotype at seroprevalence 0%. Simulation 2: Effects of temperature and the fluctuation of population immunity with a single serotype. Simulation 3: Effects of temperature and population immunity with 4 serotypes. The seroprevalence of dengue antibodies in the Singaporean population was estimated to be 70% in 1980, 60% in 1990, and 50% in 2000 [26]. Threshold MPP is the number of female mosquitoes per person that causes an epidemic (10% increase of seroprevalence). A lower threshold MPP indicates a higher probability of epidemics.

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Figure 3.

Threshold mosquito density at various population immunity levels and numbers of serotypes.

Simulations were conducted at a constant temperature of 25°C. Threshold MPP is the number of female mosquitoes per person that causes an epidemic (10% increase of seroprevalence). A lower threshold MPP indicates a higher probability of epidemics.

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Figure 4.

Comparison of the threshold mosquito density resulting in R0 = 1.

Dark green bars represent m calculated by equation 2 [16] and light green bars represent our result (MPPR0 = 1).

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Table 2.

Result of uncertainty analysis.

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Table 2 Expand

Figure 5.

Change in the threshold mosquito density.

The effect of a 5% change in each parameter after univariate sensitivity analysis.

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