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Figure 1.

Linearity of both extraction methods in conjunction with Real-time RT-PCR assay.

A 10-fold dilution series of influenza virus stock (105.7 TCID50)/ml were extracted in triplicate for each dilution with the Maxwell 16-S and QIAamp Kit and then tested by the CDC Flu A Real-time RT-PCR assay. The virus concentration (log10TCID50/ml) was plotted against the mean Ct values of triplicate determinations for each dilution producing repeated positive results.

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Figure 1 Expand

Table 1.

Analytical sensitivity and precision comparisons of Maxwell 16-S and QIAamp method.

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Table 1 Expand

Table 2.

Comparison between Maxwell 16-S and QIAamp method for RNA extraction from throat swabs and BALFsa.

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Table 2 Expand

Table 3.

Comparison of Maxwell 16-S, Maxwell 16-M and QIAamp method for extraction of influenza virus RNA from pooled fecal samplesa.

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Table 4.

Comparison of time and cost between the manual and automated extraction method.

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Table 4 Expand