Figure 1.
Sin Nombre hantavirus (SNV) RNA levels for (a) donor deermice before and during experiment 4, and (b) transmission event (TE) deermice at time zero (T0) and during experiment 4.
a) We recovered the remains of the original donor (D5a) in enclosure 5 on week 4, and substituted a new donor (D5b). Insufficient sample was collected from donor 3 on 8/1/08 for qRT-PCR analysis. Viral RNA was quantitated from all blood samples collected starting at initial capture from the wild (QT) until the end of the experiment. b) E1, E4, and E3 are the enclosures in which each TE deermouse became infected. T0 represents the last blood sample negative for both SNV RNA and antibody to SNV before SNV RNA was first detected. Testing blood samples included retesting the initial SNV-positive sample (as indicated by prior serology or nested RT-PCR) and all subsequent blood samples, as well as blood collected at 2 or more timepoints before the initial positive test. SNV RNA quantities are proportional (see Methods), not actual copy numbers.
Figure 2.
SNV antibody titers in all TE deermice in experiment 4.
E1, E4, and E3 are enclosures in which each TE deermouse became infected. T0 represents the last blood sample negative for both SNV RNA and antibody to SNV before antibody or RNA was detected.
Figure 3.
SNV RNA levels and SNV antibody titers for TE deermice with the longest time course of infection.
T0 represents the last blood sample negative for both SNV RNA and SNV antibody before SNV RNA or antibody was detected.
Figure 4.
Median number of new wounds per individual deermouse by infection status.
Thick horizontal line is the median; top and bottom of boxes represent the 25 and 75 percentile, respectively; horizontal lines at ends of dashed lines represent the minimum and maximum values, excluding one outlier (black dot). The infected category includes all donor and TE deermice from all 4 experiments. The uninfected category includes all susceptible deermice that never seroconverted. Each deermouse is represented only once in the analyses.
Table 1.
Experimental design for Sin Nombre hantavirus (SNV) transmission experiments in North American deermice (Peromyscus maniculatus) in outdoor enclosures in Montana.
Table 2.
Experimental details for experiment 4.
Table 3.
Small (S) and medium (M) segment sequence identities at the nucleotide level for SNV variants infecting donors from experiment 4.