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Figure 1.

Scanning electron micrographs of the oral sucker, ventral sucker and the posterior segment of the gynecophoral canal of male worms derived from water buffalo and from yellow cattle.

BS, border spine; SC, surface crest; SP, sensory papillae.

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Figure 2.

Scanning electron micrographs of the oral sucker, ventral sucker, genital pore (arrowheads) and the middle of the integumentum of female worms derived from water buffalo and yellow cattle.

S, spine; SP, sensory papillae; TC, tegumental crest.

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Figure 3.

Transmission electron micrographs of male schistosomes derived from water buffalo and yellow cattle.

(A,B) tegument, 2000×; (C,D) subtegument and inner structure, 12000×. CM, circular muscle; L, lipid droplet; LM, longitudinal muscle; P, pore; R, ridge; V, vacuole.

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Figure 4.

Transmission electron micrographs of female schistosomes derived from water buffalo and yellow cattle.

(A,B) tegument, 2000×; (C,D) subtegument and inner structure, 12000×. L, lipid droplet; MV, microvilli; P, pore; R, ridge.

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Figure 5.

Transcription profile analysis of significant differentially expressed genes in Schistosoma japonicum from water buffalo (Group B) and yellow cattle (Group C).

(I) Hierarchical clustering using genes (probe sets) showing differential expression (ANOVA p<0.05); (II) Principal component analysis of transcript profiles from Groups B and C.

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Table 1.

Details of real-time RT-PCR primers and confirmation of results.

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Table 2.

Selected genes overexpressed in schistosomes from water buffalo compared with those from yellow cattlea.

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Table 3.

Selected genes underexpressed in schistosomes from water buffalo compared with those from yellow cattle.

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Figure 6.

Distribution of gene ontology (GO) terms for the differentially expressed genes in schistosomes from water buffalo and yellow cattle.

Pie chart showing the GO classification for biological processes (I), molecular functions (II) and cellular components (III). The chart does not contain those genes that do not have assigned GOs.

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Table 4.

KEGG pathway analysis of differentially expressed genes in schistosomes from water buffalo compared with those from yellow cattle.

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