Figure 1.
Full MS scans of WT (A) and fbn4.
KD (B) chloroplast lipid extracts show similar compositions of the two samples. Figure shows raw data that have not been normalized to retinol.
Figure 2.
Full MS scans of WT (A) and fbn4 KD (B) plastoglobule lipid extracts show some differences between samples.
Asterisks indicate species with differences between panels (A) and (B). Figure shows raw data that have not been normalized to retinol.
Table 1.
Selected analytes that were more abundant in fbn4 KD plastoglobules compared to wild-type plastoglobules in a full-scan LC-MS.
Figure 3.
(A) MS/MS analysis and possible structure. Abundance in WT and fbn4 KD lipid extracts from chloroplasts (B) and plastoglobules (C), normalized to retinol. Data are means ± SD of three measurements; *, P<0.05 using Student's t test. Similar results were obtained in two biological replicates for the chloroplasts and three biological replicates for the plastoglobules.
Figure 4.
(A) MS/MS analysis. Abundance in WT and fbn4 KD lipid extracts from chloroplasts (B) and plastoglobules (C), normalized to retinol. Data are means ± SD of three measurements; *, P<0.05 using Student's t test. Similar results were obtained in two biological replicates for the chloroplasts and three biological replicates for the plastoglobules.
Figure 5.
Abundance in WT and fbn4 KD lipid extracts from chloroplasts (B) and plastoglobules (C), normalized to retinol. Data are means ± SD of three measurements; *, P<0.05 using Student's t test. Similar results were obtained in two biological replicates for the chloroplasts and three biological replicates for the plastoglobules.
Figure 6.
Schematic representation of chloroplast and plastoglobule extraction process from apple leaves.
Figure 7.
Transmission electron micrographs of extracted plastoglobules of WT and fbn4 KD apple tree leaves.
Leaves were harvested from 4 to 5 month-old trees grown under 90 µE m−2 s−1 light intensity with a 12 h photoperiod. Extracted plastoglobules were stained with OsO4 and observed on a copper mesh support. Transmission electron micrographs of electron opaque (black) and electron transparent (white) plastoglobules extracted from WT (upper row) and fbn4 KD (lower row) are shown.