Figure 1.
Phage AP22 plaques with opaque haloes on the host A. baumannii 1053 strain.
Figure 2.
Infection curves of phage AP22.
Representative curves are based on results from three independent assays of a non-infected A. baumannii culture and cultures infected at MOI of 0.5, 5 and 50. Standard deviations are indicated.
Figure 3.
AFM images of bacteriophages AP22.
AFM height images (A, D), section analysis (B, E) and height distribution histograms (C, F) of bacteriophages AP22 deposited on the mica (top row) and HOPG (bottom row) surfaces. Sections are built along the dotted lines on the corresponding AFM images. Insets in A and D demonstrate zoomed bacteriophages from the same image. Top inset in A is presented in deflection channel to emphasize holes in the center of the phages.
Figure 4.
AFM images of A. baumannii cells.
AFM height (A) and deflection (B) images of A. baumannii cells deposited on the mica surface. (C) Section analysis built along the horizontal line in (A). (D) Zoomed-in region of A. baumannii surface. The inset in (B) demonstrates zoomed-in region around bacterial aggregate (shown by the white square) with the EPS and pili.
Figure 5.
AFM images of A. baumannii cells infected for 1–6 minutes.
Left row: deflection AFM images of A. baumannii cells incubated with bacteriophages AP22 for 1 (A), 3(C), 6 (E) minutes. Right row: zoomed-in three dimensional reconstructions of bacterial surfaces depicted on the left (the zoomed regions are indicated by dotted circles in the height images). The inset in (A) demonstrates zoomed-in region, shown by the white square.
Figure 6.
AFM images of A. baumannii cells infected for 15–60 minutes.
AFM deflection (A, D) and height (G) images of A. baumannii cells incubated with bacteriophages AP22 for 15 (A), 30 (D), 60 (G) minutes. (B), (E) and (H) represent the section analysis along the dotted lines on (A), (D) and (G) correspondingly. (C), (F) and (I) – zoomed-in three dimensional reconstructions of bacterial surfaces depicted on the left.
Figure 7.
The density of adsorption of bacteriophages AP22.
The density of adsorption of bacteriophages AP22 on the surfaces of mica and bacteria for the samples incubated during different time periods.