Table 1.
Sampling dates, initial seawater characteristics and incubation temperatures.
Figure 1.
Relative abundance of bacterial classes in the starting communities (seawater fraction <10 µm).
The chart was constructed based on the relative abundances of OTUs (16S ribosomal amplicon pyrosequencing) in the standardized subsample (n = 494 sequences). Classes represented by less than 2% of sequences are summarized under “others”.
Table 2.
PERMANOVA main tests of bacterial community composition based on Jaccard dissimilarities of ARISA profiles.
Table 3.
PERMANOVA pair-wise comparisons of bacterial community composition based on Jaccard dissimilarities of ARISA profiles.
Table 4.
PERMANOVA main tests of bacterial community composition based on Bray-Curtis dissimilarities of OTUs (16S ribosomal amplicon pyrosequencing; ‘no dilution’ and ‘serial dilution’).
Table 5.
PERMANOVA pair-wise comparisons of bacterial community composition based on Bray-Curtis dissimilarities of OTUs (16S ribosomal amplicon pyrosequencing; ‘no dilution’ and ‘serial dilution’).
Table 6.
PERMANOVA main tests of bacterial community composition based on Bray-Curtis dissimilarities of OTUs (16S ribosomal amplicon pyrosequencing; ‘initial dilution’ summer).
Figure 2.
Bacterial groups in the ‘season’-‘dilution’ combinations, based on SIMPER analysis of pH in situ levels.
Displayed are phylogenetic groups jointly contributing to 90% of the total similarity within and dissimilarity between pH in situ levels of the different dilution treatments, separately for each season (no: ‘no dilution’, serial: ‘serial dilution’, initial: ‘initial dilution’). The heat map summarizes the contributions of single OTUs (16S ribosomal amplicon pyrosequencing) on the family level. Av.Si%: average percentage contribution of the i th species to the total similarity, Av.δi%: average percentage contribution of the i th species to the total dissimilarity. Bacterial families contributing less than 1% to both totals are summarized under “others”. The amount of contribution is indicated by the color of cells, darker colors represent higher contributions.
Figure 3.
Bacterial groups responding differently to pH, based on SIMPER analysis.
Displayed are phylogenetic groups jointly contributing to 90% of the total similarity within and dissimilarity between pH levels, separately for each ‘season’-‘dilution’ combination (no: ‘no dilution’, serial: ‘serial dilution’, initial: ‘initial dilution’). The heat map summarizes the contributions of single OTUs (16S ribosomal amplicon pyrosequencing) on the family level. Av.Si%: average percentage contribution of the i th species to the total similarity, Av.δi%: average percentage contribution of the i th species to the total dissimilarity. Bacterial families contributing less than 1% to both totals are summarized under “others”. The amount of contribution is indicated by the color of cells, darker colors represent higher contributions.