Figure 1.
Chemical structures of the siderophores pyochelin, watasemycin A, yersiniabactin, enterobactin and citrate.
Table 1.
MIC of pyochelin against selected bacterial strains.
Table 2.
Influence of iron on the MIC of pyochelin.
Table 3.
MIC of pyochelin in the presence of selected additives.
Figure 2.
Levels of reactive oxygen species in E. coli wild type and entE mutant.
Quantitation of ROS levels using the DCFA-DA probe. Fluorescence intensities are relative to that of the control. Control: wt grown in 2 mL M9 medium; PCH indicates cells grown in the presence of 15 µM pyochelin; ASC indicates supplementation with 1 mM ascorbic acid; ENT designates addition of 1 µM of pure enterobactin; CIT indicates supplementation with 50 µM citrate. Error bars = SEM, n = 3.
Table 4.
Growth inhibition by pyochelin under aerobic and anaerobic culture conditions.
Table 5.
List of strains used in this work.