Figure 1.
Distribution of bacterial orders observed in 16S rRNA gene clone libraries of 12C vs. 13C fractions from 13C-NaHCO3 incubations at NH-10 in September 2008 and March 2009.
Figure 2.
Class distributions of 16S rRNA gene clone libraries of 13C fractions from all 13C-NaHCO3 incubations at NH-10 in September 2008, March 2009, and May 2010, as well as at LP-6 and CR-20 in May 2010.
Figure 3.
Rooted neighbor-joining phylogenetic tree of Alphaproteobacteria species actively involved in DIC assimilation obtained from 16S rRNA gene clone libraries of 13C fractions from SIP experiments conducted at NH-10 in September 2008, March 2009, and May 2010. Clones representing highly similar OTUS were collapsed for clarity.
Figure 4.
Rooted neighbor-joining phylogenetic tree of Alphaproteobacteria involved in DIC assimilation obtained from 16S rRNA gene clone libraries of 13C fractions from SIP experiments conducted at NH-10, CR-20, and LP-6 in May 2010. Clones representing highly similar OTUS were collapsed for clarity.
Figure 5.
Rooted neighbor-joining phylogenetic tree of Gammaproteobacteria involved in DIC assimilation obtained from 16S rRNA gene clone libraries of 13C fractions from SIP experiments conducted at NH-10 in September 2008, March 2009, and May 2010.
Clones representing highly similar OTUS were collapsed for clarity. Shaded clades represent those identified by Swan et al., 2012 to contain RubisCo and possibly participate in dark carbon fixation.
Figure 6.
A. Rooted neighbor-joining phylogenetic tree of Gammaproteobacteria involved in DIC assimilation obtained from 16S rRNA gene clone libraries of 13C fractions from SIP experiments conducted at NH-10, CR-20, and LP-6 in May 2010.
Highly similar OTUS were collapsed for clarity. Shaded clades represent those identified by Swan et al., 2012 to contain RubisCo and possibly participate in dark carbon fixation.
Figure 7.
Rooted neighbor-joining phylogenetic tree of Bacteroidetes involved in DIC assimilation obtained from 16S rRNA gene clone libraries of 13C fractions from SIP experiments conducted at NH-10 in September 2008, March 2009, and May 2010.
High similar OTUs were collapsed for clarity.
Figure 8.
Rooted neighbor-joining phylogenetic tree of Bacteroidetes involved in DIC assimilation obtained from 16S rRNA gene clone libraries of 13C fractions from SIP experiments conducted at NH-10, CR-20, and LP-6 in May 2010.
High similar OTUS were collapsed for clarity.
Figure 9.
Rooted neighbor-joining phylogenetic tree of Deltaproteobacteria, Betaproteobacteria, Verrucomicrobia, Actinobacteria, and Planctomycetes involved in DIC assimilation obtained from 16S rRNA gene clone libraries of 13C fractions from SIP experiments conducted at NH-10 in September 2008, March 2009 and May 2010.
High similar OTUs were collapsed for clarity. Shaded clades represent those identified by Swan et al., 2012 to possibly participate in C-1 metabolism or dark carbon fixation.
Figure 10.
Rooted neighbor-joining phylogenetic tree of Deltaproteobacteria, Betaproteobacteria, Verrucomicrobia, Actinobacteria, and Planctomycetes involved in DIC assimilation obtained from 16S rRNA gene clone libraries of 13C fractions from SIP experiments conducted at NH-10, CR-20, and LP-6 in May 2010.
High similar OTUs were collapsed for clarity. Shaded clades represent those identified by Swan et al., 2012 to possibly participate in C-1 metabolism or dark carbon fixation.