Figure 1.
Dioxin and control lineage F1 and F3 generation adult-onset kidney disease.
Percentages of females (panel A) and males (panel B) with kidney disease and number of diseased rats/total number of rats (*P<0.05). Micrographs (Scale bar = 200 µm) show kidney disease in F3 generation dioxin lineage (panel E and F) compared to control (panel C and D) for female (panel C and E) and male (panel D and F).
Figure 2.
Dioxin and control lineage F1 and F3 generation pubertal abnormality and ovarian disease.
Percentages of females (panel A) and males (panel B) with pubertal abnormality, or primordial follicle loss (panel C), or polycystic ovary disease (panel D), or those with tumor development (panels E and F). The number of diseased rats/total number of rats in each lineage are presented (*P<0.05; ***P<0.001).
Figure 3.
Dioxin and control lineage F1 and F3 generation adult-onset transgenerational testis or prostate disease.
Percentages of males with testis (panel A) or prostate disease (panel B) and number of diseased rats/total number of rats (***P<0.001). Micrographs (Scale bar = 200 µm) show testis and prostate disease in F3 generation dioxin lineage (panels D and F) compared to control (panels C and E).
Figure 4.
Dioxin and control lineage F1 and F3 generation adult-onset diseases in rats.
Incidences of total female disease (panel A), total male disease (panel B), female multiple disease (panel C) and male multiple disease (panel D) and number of diseased rats/total number of rats (*P<0.05; **P<0.01; ***P<0.001).
Table 1.
Sperm differential methylation regions (DMR) in F3 generation dioxin lineage.
Figure 5.
Dioxin promoted F3 generation sperm epimutations.
Chromosomal locations for transgenerational differential DNA methylation regions (DMR) (arrowheads). There were 50 DMR in sperm DNA from dioxin lineage rats compared to control lineage rats.
Figure 6.
Dioxin induced DMR and functional gene categories.
Number of DMR associated with various gene categories.
Figure 7.
Gene network of DMR associated genes.
The DMR associated genes with connections to various cellular processes and associated cellular localization.