Figure 1.
Microarray experimental design for investigating DDT resistance in An. arabiensis from a polluted breeding site in Dioulassoba.
Gene expression profiles of mosquitoes from Dioulassoba exposed (DSSBA RES) and unexposed (DSSBA CON) to DDT (4%) were compared to a laboratory An. arabiensis strain (MOZ SUS).
Table 1.
The genotypes at the 1014 kdr locus in An. arabiensis from Dioulassoba unexposed and exposed to DDT 4% in WHO susceptibility tests.
Figure 2.
Venn diagram showing the pattern of differential expression among the microarray comparisons.
Microarray probes were considered significantly expressed at p<0.05 after the application of a multiple testing correction (Benjamini-Hochberg).
Table 2.
The most overrepresented biological terms associated with probes significantly differentially expressed in the microarray experiments comparing field An. arabiensis populations from Burkina Faso (DSSBA RES and DSSBA CON) and an insecticide-susceptible strain from Mozambique (MOZ SUS).
Table 3.
P450s significantly expressed between the Dioulassoba populations (DSSBA RES and DSSBA CON) and MOZ SUS.
Table 4.
Differentially expressed glutathione-S-transferases between DSSBA RES and DSSBA CON.
Table 5.
The ten differentially expressed probes common in all three microarray comparisons.
Figure 3.
Fold-change in GSTD3 expression for microarray comparisons using quantitative qPCR.
Fold changes are expressed using the 2−ΔΔCt formula with 95% C.I. [39].