Figure 1.
Confocal microscopy: (A-D) Confocal microscopy autofluorescence images of 20 µm ADM cryocuts, scale bars equal (A,C) 75 µm, (B,D) 50 µm; (D) the white arrow indicates a putative vessel channel; (E,F) autofluorescence images of 10 µm cryocuts of native human skin, scale bars equal (E,F) 75 µm.
Figure 2.
Confocal microscopy overlays of autofluorescence scans (green) and the respective anti- matrix antibody staining (blue); comparison of 20 µm cryocuts of ADM (A,C,E,G,I,K) and 10 µm cryocuts of native human skin (B,D,F,H,J,L); scale bars equal 150 µm.
(A,B) anti- collagen I (C,D) anti- collagen III (E,F) anti- collagen IV (G,H) anti- laminin 1 (I,J) anti- fibronectin (K,L) anti- hyaluronic acid.
Table 1.
Summary of immunostaining with human antibodies against matrix components of the ADM; + means detectable by immunostaining, - means absence of any detectable signal.
Figure 3.
Confocal microscopy overlays of autofluorescence scans (green) and PI staining (orange), 20 µm cryocuts, scale bars equal 150 µm.
(A) only PI (B) treatment with DNAse+PI (C) treatment with RNAse+PI (D) double treatment with DNAse+RNAse+PI
Figure 4.
DNA content from 8 different donors.
Figure 5.
Gelelectrophoresis - DNA from 8 different donors, as DNA marker a 100bp DNA ladder was used.
Figure 6.
Confocal microscopy overlays of autofluorescence scans (green) and the respective positive cell surface and intracellular antibody (red) staining, 20 µm cryocuts, scale bars equal (A,B,C) 50 µm, (D) 150 µm.
(A) Anti. α-Catenin (B) Anti- Cytokeratin (C) Anti- vWF (D) Anti- VEGFR1
Table 2.
Biomechanical results of the two available sizes thin and thick of the ADM; Values are presented as mean (SD), P- Value evaluate significances between the differences of properties of the different sizes.