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Figure 1.

SLO fundus image of a venous junction.

Angle is determined from the slope of the tangent to the vessel at the measured location. wsl,x, wsl,y; image width in x- and y-direction. DP; detection plane.

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Figure 1 Expand

Figure 2.

Flow chart for bidirectional Doppler FD-OCT.

Flow chart explaining velocity extraction based on bidirectional Doppler FD-OCT.

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Figure 3.

Geometrical situation at the posterior pole of the eye.

and , probe beam wave vectors; , velocity vector; and , Doppler angles; , separation angle between probe beams; , angle between and the plane perpendicular to the detection plane; , change in illumination angle due to eye movement.

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Table 1.

Baseline blood pressure, pulse rate and vessel diameters at the two study days.

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Table 1 Expand

Table 2.

Velocity, diameter and blood flow data under baseline conditions and during 100% oxygen breathing measured on Doppler OCT or Laser Doppler Velocimetry study day.

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Table 2 Expand

Figure 4.

Correlation analysis for velocity measurements.

Correlation between velocity and blood flow as assessed with LDV and OCT, respectively, during baseline conditions and during hyperoxia.

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Figure 5.

Bland-Altman of velocity measurements.

Bland-Altman plot comparing velocity data as obtained with laser Doppler velocimetry and with bidirectional Doppler FD-OCT. Data are presented for baseline conditions as well as for conditions during systemic hyperoxia.

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Figure 6.

Bland-Altman plot of blood flow calculation.

Bland-Altman plot comparing retinal blood flow data as calculated from measurements of blood flow velocity obtained either with laser Doppler velocimetry or with bidirectional Doppler FD-OCT combined with diameter measurements with the Dynamic Vessel Analyzer. Data are presented for baseline conditions as well as for conditions during systemic hyperoxia.

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Figure 7.

Phase tomograms at baseline and during hyperoxia.

Sample OCT measurements as obtained in both channels during baseline conditions (A) and during breathing 100% oxygen (B). Vasoconstriction and reduction of velocity can be observed in the lower tomograms.

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Figure 8.

Sample phase extraction of a baseline measurement.

Over the measurement period of 12 s the eye moved relative to the incoming laser beams resulting in pronounced changes in Ф1 and Ф2, but almost unchanged ΔФ.

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Figure 9.

Sample phase extraction of a baseline measurement.

Over the measurement period of 12 s several small eye movements can be seen resulting in pronounced changes in Ф1 and Ф2, but almost unchanged ΔФ. In addition, the eye also shows a slight overall movement resulting in shifts of Ф1 and Ф2 over time.

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Figure 10.

Calculation of angle .

Angle as calculated from measurements using OCT (red) and LDV (blue) from the two channels. Calculations were done at baseline conditions as well as during hyperoxia.

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