Figure 1.
Reported distribution of the Rasberry Crazy Ant in the United States (in blue).
The distribution of Nylanderia cf. pubens in Florida is given in orange, but we suspect that these may prove to be N. fulva. Counties highlighted with solid colors indicate verified occurrences, whereas hatched counties are unconfirmed reports. Red dots indicate collection sites for samples used in this study. The actual distribution of N. fulva in the United States is most likely to be more widespread.
Table 1.
Species identification and collection localities of samples of N. pubens, N. fulva, and the RCA/N. cf. pubens used in this study.
Figure 2.
Results of the discriminant analysis of principal components (DAPC) of 14 morphometric measurements.
Species are color-coded. a) Scatter plot of the first two discriminant functions based on k-means clustering. Variable contribution (>10% loading) to the first axis is primarily scape length, profemur length, gaster length, total length, and Weber’s length, whereas gaster length, mesonotum setae count, and total length contribute to the second axis. b) Heat map of assignment probabilities of individual samples (rows; order identical to Table S1) to species (columns) based on discriminant functions. Blue crosses indicate group membership as assigned by the authors (i.e. species membership). Cluster assignment based on k-means clustering is shown by red rectangles (lower right: clusters 1+2; upper left: clusters 3+4).
Figure 3.
Phylogenetic tree estimation of six concatenated loci.
Shown is the maximum likelihood phylogeny. Branch support values (maximum likelihood bootstrap and Bayesian posterior probabilities [BS/PP]) are shown except on the very short branches. The inset shows the unresolved Bayesian analysis for the Nylanderia fulva complex. Nylanderia fulva samples are shown in blue, N. pubens sample are indicated in orange.
Figure 4.
A) worker of Nylanderia pubens in lateral view; B) male N. fulva paramere in lateral view; C) male N. pubens paramere in lateral view.