Figure 1.
Evolution of Fraction of Transpirable Soil Water (FTSW) during water deprivation.
Each line reflects the average values of three values for each genotype either under FDS or under FIS. Vertical dotted line indicates the date of the FDS tissue collection. Horizontal dotted line indicates the FTSW level at which FIS collection was carried out. Triangles correspond to treated plants whereas circles correspond to their untreated counterparts. Genotypes are color-coded as follows: Inedi (black), Tekny (gray), Melody (red), SF109 (turquoise), SF326 (yellow), SF193 (magenta), SF028 (green) and SF107 (blue).
Figure 2.
Dendograms and heatmaps of genes and individuals both under FDS (A) and FIS (B).
Triangles correspond to treated plants whereas circles correspond to their untreated counterparts. Genotypes are color-coded as follows: Inedi (black), Tekny (gray), Melody (red), SF109 (turquoise), SF326 (yellow), SF193 (magenta), SF028 (green) and SF107 (blue).
Table 1.
Genes showing ANOVA effects for two drought scenarios.
Table 2.
GO term enrichment test results for genes showing ANOVA effects for two drought scenarios.
Figure 3.
Results of the sparse Partial Least Squares (SPLS) analysis on plants under FDS (A) and FIS (B).
Plots show respectively the repartition of the morphophysiological variables (left) and individuals (right) along the first two components of the SPLS. Morphophysiological variables are carbon isotope discrimination (CID), collar diameter (CoD), Transpiration rate (E), Integrated Transpired Water (ITW), Leaf Mass per Area (LMA), Osmotic Potential (OP), Plant Height (PHe), Relative Water Content (RWC) and Total plant Leaf Area (TLA). Triangles correspond to treated plants whereas circles correspond to their untreated counterparts. Genotypes are color-coded as follows: Inedi (black), Tekny (gray), Melody (red), SF109 (turquoise), SF326 (yellow), SF193 (magenta), SF028 (green) and SF107 (blue).
Figure 4.
Gene-Phenotype network produced by SPLS, based on responses of eight sunflower genotypes to two drought stress scenarios implemented in controlled environment.
Genes presenting absolute correlation scores higher than 0.65 with at least one morpho-physiological variable are represented. Each circle represents one gene. Blue, red and purple edges indicate, respectively, whether the gene-phenotype association exists under FDS, FIS or both stress scenarios. Each gene circle is split in three slices displaying ANOVA results. Yellow, red and black slices represent, respectively, treatment effect under FDS (moderate stress responsive genes), treatment effect under FIS (severe stress responsive genes), and g*t effect under FIS (gene likely to explain genotypic differences in stress responses). Numbers of genes for each combination of ANOVA effects are shown for each gene-phenotype group. Phenotypic responses are in gray squares, OP: Osmotic Potential, LMA: Leaf Mass Area, E: Transpiration Rate, RWC: Relative Water Content, ITW: Integrated Transpired Water, PHe: Plant Height.
Figure 5.
Gene-Phenotype sub-network produced by SPLS, based on responses of eight sunflower genotypes to two drought stress scenarios implemented in controlled environment.
Only genes regulated by drought stress in field conditions are shown. Each ellipse represents one gene. Blue, red and purple edges indicate, respectively, whether the gene-phenotype association exists under FDS, FIS or both stress scenarios. Sunflower Heliagene cluster IDs are shown when meaningful names of Arabidopsis homologs are not available. Gray squares represent phenotypic responses; OP: Osmotic Potential, E: Transpiration Rate, RWC: Relative Water Content, ITW: Integrated Transpired Water, PHe: Plant Height.