Figure 1.
Transmission electron microscopy of E. tarda strains.
A: Electron micrographs of 10,000× magnification; B: Electron micrographs of 100,000× magnification to show flagella filaments.
Table 1.
Characterization of E. tarda strains.
Figure 2.
Growth curve of E. tarda strains.
y axis: OD540 values of E. tarda (Log 10); x axis: incubation time.
Figure 3.
ECP profiles of E. tarda strains.
M: Protein molecular weight marker, 1: H1, 2: ΔfliC mutant, 3: fliC+. The major bands are shown by arrows from top: 52 kDa, 22 kDa, 18 kDa.
Table 2.
Bacterial strains and plasmids used in this study.
Table 3.
Primers used for fliC deletion and complementation.