Table 1.
Expression of type I interferon and IL-6 in AEC co-cultured with MDDC.
Table 2.
Chemokine family members overexpressed by AEC-conditioned DC.
Figure 1.
Airway epithelial cell-induced changes in DC expression of chemokine genes.
After 5 days of culture in the presence or absence of AEC, DC were sorted by flow cytometry. RNA from 15 independent experiments was extracted, and expression of chemokine genes was determined using quantitative real-time PCR. *** p<0.001.
Figure 2.
Airway epithelial cell-induced changes in DC expression of complement family genes.
After 5 days of culture in the presence or absence of AEC, DC were sorted by flow cytometry. RNA from 15 independent experiments was extracted, and expression of complement pathway genes determined using quantitative real-time PCR. **p<0.01; ***p<0.001.
Figure 3.
Airway epithelial cell-induced changes in DC expression of Fcγ receptor genes.
After 5 days of culture in the presence or absence of AEC, DC were sorted by flow cytometry. RNA from 15 independent experiments was extracted, and expression of Fc gamma receptor genes was determined using quantitative real-time PCR. **p<0.01; ***p<0.001.
Figure 4.
Airway epithelial cell-induced changes in DC expression of selected immune response genes.
(A). After 5 days of culture in the presence or absence of AEC, DC were sorted by flow cytometry. RNA from 15 independent experiments was extracted, and expression of immune response genes was determined using quantitative real-time PCR. **p<0.01; ***p<0.001. (B) Cell surface expression of B7-H1 and ICAM-1 was determined by flow cytometry. Cells staining with specific antibody and isotype control antibodies are shown. Histograms from a representative experiment are shown. Similar changes were seen in all 8 experiments performed.
Figure 5.
DC and airway epithelial cell expression of CD200R1 and CD200.
After 5 days of culture in the presence or absence of AEC, cell surface expression of CD200R1 on DC and CD200 on AEC was determined by flow cytometry. Histograms from a representative experiment are shown. Similar changes were seen in all 6 experiments performed.