Table 1.
28 core genes of rhizobia used in this study.
Table 2.
Primers used in this study.
Figure 1.
The maximum likelihood (ML) phylogenetic trees.
The tree was constructed by PhyML based on concatenated sequences of 28 core genes (A), SMc00019-truA-thrA concatenate (B) or recA-glnII-atpD concatenate (C). Bootstrap confidence levels ≥50% are indicated at the internodes. Bar = 10% nucleotide divergence.
Figure 2.
Plotted results of ANIstt (average nucleotide identity of SMc00019-truA-thrA) versus ANIm (genomic ANI).
460 intra-genus pairwise comparisons in Bradyrhizobium (190), Mesorhizobium (3), Sinorhizobium (91), Rhizobium (10), Agrobacterium (6), Cupriavidus (10), Methylobacterium (28), Ralstonia (10), Rhodopseudomonas (21) and Burkholderia (91). 7/460 points lie between the interspecies region (ANIm <93% and ANIstt <94%) and the intraspecies region (ANIm >95% and ANIstt >96%). The thick and fine solid line(s) depicted the quadratic curve (the best curve to fit the data) and the individual confidence intervals (95%). The quadratic equation and R square are shown.