Figure 1.
Alkaline Comet, neutral Comet and Sperm Chromatin Dispersion for total fertile donors, for low dsSDF and high dsSDF fertile donor subgroups and for the unexplained RPL group.
Table 1.
Sperm DNA fragmentation (mean ± SD) in fertile donors and unexplained RPL samples.
Figure 2.
Pulsed-field gel electrophoresis of semen samples DNA from fertile donors (A, lanes 1 and 2; B, lane 1), negative control (B, lane 1), positive controls with DNAse 0.5 mg/ml, 30 minutes (B, lanes 2, 3 and 4) and RPL samples (B, lanes 5, 6 and 7).
DNA molecular weight markers consisting of Low Range PFG Marker (M1) and Lambda ladder PFG marker (M2) are detailed. Negative controls in B, lane 1 show a thin compression zone. Positive controls in B, lanes 2, 3 and 4 show DNA digestion into sizes of around the 48 Kb. Sperm DNA fragmentation of the specific samples of this figure is shown in Table 2.
Table 2.
Relation of samples shown in Figure 2 with their sperm DNA fragmentation.
Figure 3.
ROC curves analysis for alkaline Comet, neutral Comet and SCD attending: Pregnancy without miscarriage (A), and recurrent miscarriage (B).
Figure 4.
Model for ssDNA and dsDNA breaks mechanisms and clinical outcomes.
ssSDF model (A) dsSDF model (B).