Table 1.
Primer sequences and cycling conditions used for amplification.
Figure 1.
Schedule showing preserved elements (in black).
Table 2.
Bone preservation and measurements.
Figure 2.
Photograph showing thoracic vertebra trauma.
Figure 3.
Photograph showing irregular bony outgrowths on both sides of the frontal crest.
Figure 4.
Sequencing chromatograms showing identical sequences of a part of HVII from the skull sample (upper chromatogram) and the formalin-fixed paraffin-embedded tissue sample used as reference.
Figure 5.
Pyrosequencing results for a part of the amelogenin gene for sex determination.
The upper pyrogram (skull sample) indicates a female individual, which can be seen by the different sequence pattern from dispensation 19 to 23 due to the six-bp deletion female individuals have in this part of the amelogenin gene. The lower pyrogram (the reference material) shows a male individual.
Table 3.
STR genotypes and mtDNA determined in the remains and the reference sample.