Table 1.
Bloom sample metadata.
Figure 1.
Recruitment plots of metagenomes to Microcystis aeruginosa NIES 843.
Recruitment plots of environmental Microcystis reads from each metagenome to the M. aeruginosa NIES 843 genome. A) Recruitment of GLSM Microcystis reads to NIES 843. B) Recruitment of Taihu Microcystis reads to NIES 843. C) Recruitment of Erie Microcystis reads to NIES 843. Position along the x-axis indicates position along the genome of M. aeruginosa NIES 843 from zero to 5.8 Mbp and position along the y-axis indicates percent similarity of recruited sequences. The bar along the bottom is a secondary indicator of percent similarity to the reference genome. See Table S2 for locations of potential MI’s within each recruitment.
Figure 2.
Metagenomic islands (MIs) of bloom-associated Microcystis.
Metagenomic islands (MIs) identified in the Lake Erie and Taihu Microcystis recruitments. Six regions in Taihu and three in Erie qualified as MIs. MIs were defined as regions of ≥10 kb in length that have an average similarity of less than 25% to the reference genome and less than 25% total coverage of the corresponding base pairs of the reference sequence. A) View of MIs of Lake Erie Microcystis recruitment. Boxes ii, iii, and vi were found to be MIs in Taihu, but did not satisfy our criteria in Lake Erie. E) View of MI regions in Taihu Microcystis recruitment.
Figure 3.
Identity of bloom-associated microbial community members.
A) Percent abundance of orders belonging to the Cyanobacteria phylum. Abundance is a percentage of reads annotated as Cyanobacteria from each metagenome. B) Distribution of bacterial phyla in each metagenome. Abundance is a percentage of total bacterial reads in each metagenome. Phyla with less than 1% total abundance were grouped in the “Other” category.
Table 2.
Percentage abundance of hits assigned to genera in the orders Chroococcales and Oscillatoriales.
Figure 4.
Abundance of metagenome reads assigned to each COG category.
Metagenomes were annotated using the COG database. Reads were assigned to standard COG functional categories based on this annotation. Abundance is a percentage of total hits to the COG database.
Figure 5.
Comparison of number of hits to COG categories between bloom metagenomes.
Normalized number of hits to each COG category from each metagenome plotted against the other. Data were normalized to the metagenome with the greatest number of hits to the COG database (Taihu). A) COG distributions of Erie and GLSM are compared. Data points largely fall within or close to the 95% confidence interval (CI) which is indicated by the black lines, indicating similarity. B) COG distributions of Erie and Taihu are compared. C) COG distributions of Taihu and GLSM are compared. Outliers in (B) and (C) are largely conserved, indicating closer conservation of COG distribution between the North American lakes to each other than Taihu. The largest outlier in the Taihu comparisons is denoted by the () symbol in each plot. This outlier corresponds to the Amino Acid Metabolism and Transport COG category.
Table 3.
The abundance of microbial nitrogen assimilation genes within metagenome.