Table 1.
Clinical and laboratory findings in five patients with Guillain-Barré syndrome from whom Campylobacter jejuni was isolated and used in the current studya.
Figure 1.
Serum samples from patients with GBS and from controls with other neurological disease (OND) and family controls (FC) were tested for IgG activity to lipo-oligosaccharides (LOS) from 6 C. jejuni.
Subcharts represent serum IgG activity to LOS from (A) C. jejuni BD-07 isolated from a GBS patient (B) C. jejuni BD-39 isolated from a GBS patient, (C) C. jejuni BD-10 isolated from a GBS/MFS patient, (D) C. jejuni BD-67 isolated from a GBS/MFS patient, (E) C. jejuni BD-94 isolated from a GBS patient (F) C. jejuni Penner HS:03 serostrain (CCUG 10937), lacking ganglioside mimics, as a control. *p<0.001. Lines () indicate cut-off value. GBS, Guillain-Barré syndrome; OND, other neurological disease; FC, family controls; LOS, lipo-oligosaccharides.
Table 2.
Clinical characteristics of GBS patients associated with presence IgM and/or IgG antibodies in serum to the gangliosides GM1, GD1a and GQ1b*.
Figure 2.
Binding of mouse monoclonal antibodies against LOS from C. jejuni BD-07 and BD-39.
DG-1 (binding to GM1), TBG-3 (binding to GD1a), EG-7 (binding to GD1b), EG-3 (binding to GQ1b) and EG-1 (binding to GQ1b/GT1a).
Figure 3.
ELISA analysis of the cross-reactivity of GBS patient’s serum anti-ganglioside antibodies to LOS from the autologous C. jejuni strains.
A) Optical density (OD) of IgG anti-GD1a reactivity in serum from patient DK-07 by pre-incubation with LOS from the autologous C. jejuni BD-07 strain (GM1/GD1a mimic, Table 3) and from Penner HS:03 serostrain (CCUG 10937). B) Optical density (OD) of IgG anti-GM1 reactivity in serum from patient DK-39 by pre-incubation with LOS from the autologous C. jejuni BD-39 strain (GM1/GD1a mimic, Table 3) and from Penner HS:03 serostrain (CCUG 10937). Graphs represent data as mean ± standard error.
Figure 4.
Proposed LOS outer core structures based on capillary-electrophoresis electrospray ionization mass spectrometry analysis of O-deacylated LOS samples (see Table S1).
(A) strains BD-07 and BD-39 show mimicry with GM1 and GD1a; (B) strains BD-10 and BD-67 show mimicry with GA2 and GD3. Terminal regions mimicking gangliosides are indicated with arrows.
Table 3.
Variants of the glycosyltransferases involved in synthesis of LOS outer core structures in C. jejuni strains BD-07, BD-10, BD-39 and BD-67a.