Figure 1.
Factors affecting success of DNA extraction from herbarium specimens.
Table 1.
Performance of different DNA polymerase enzymes tested.
Figure 2.
Effect of extraction method on DNA yield and purity.
Table 2.
Performance of different DNA extraction methods with historic plant herbarium specimens; results of the three best performing methods are in bold under each category.
Figure 3.
Effect of extraction method on PCR success as measured by number of positive amplicons. RM = room temperature.
Table 3.
Variation in amplification success between the three sequencing regions used.
Figure 4.
Effect of sample preparation method on PCR success (%), measured as the number of positive amplicons divided by the total number of samples.
Table 4.
Performance of different methods of specimen preparation in relation to DNA extraction success.
Table 5.
Regions and primers used for testing DNA quality through PCR amplification.