Figure 1.
P. gingivalis fim gene cluster.
We constructed a mutant with the whole region of the fim gene cluster from fimX through fimE deleted. The schema was drawn on the basis of ATCC 33277. However, genome-analyzed strains of W83 and TDC60 show that they are the same as that of ATCC 33277 in the gene arrangement.
Figure 2.
Immunoblot analysis for FimA using whole-cell sonicates.
Whole-cell sonicates were denatured in an SDS-containing buffer with 2-mercaptoethanol by heating at 100°C (upper) and 80°C (lower) for 10 min, then subjected to SDS-PAGE and immunoblot analysis. A mixture of specific antisera to the FimA polymer and monomer was used. Antigen samples were as follows: P. gingivalis ATCC 33277 Δmfa1 (expresses native FimA fimbriae, lane 1), P. gingivalis ATCC 33277 Δmfa1 Δfim cluster (FimA deficient, lane 2) with fimX-pgmA-fimA (lane 3), pgmA-fimA (lane 4), fimA (lane 5), fimX & fimA (lane 6) complementarily introduced, and P. gingivalis W83 Δfim cluster with fimA introduced (lane 7). All introduced genes originated from P. gingivalis ATCC 33277. Incomplete dissociation of FimA polymers produces a ladder-like band indicating oligomers in the lower panel. Note that bands slightly higher than 60 kDa (the lower panel) are dimers although monomers appear to be about 40 kDa (the upper panel).
Figure 3.
Transmission electron microscopic observation of FimA fimbriae on the bacterial cell surface.
P. gingivalis ATCC 33277 Δmfa1 Δfim cluster cells with fimA from 33277, TDC60, 6/26, W83, HG564 and HNA99 introduced by using an expression vector. Samples were negatively stained with 1% ammonium molybdate. Arrows indicate fimbrial structure. Some fimbriae appear to be bundled. Bars show 0.2 µm.
Figure 4.
Immunoblot analysis for PgmA using whole-cell sonicates.
Whole-cell sonicates (W) were fractionated into soluble (Sol), envelope (Env), inner membrane (IM) and outer membrane (OM) fractions. Samples were denatured in an SDS-containing buffer with 2-mercaptoethanol by heating at 100°C for 10 min, then subjected to SDS-PAGE and immunoblot analysis. Emp denotes 33277 Δmfa1 Δfim cluster/pT-COW::ragAP, carrying empty vector, used as a negative control; 33277 denotes the wild-type strain; Complement denotes 33277 Δmfa1 Δfim cluster carrying pT-COW::ragAP::fimX-pgmA-fimA. An arrow indicates PgmA as a 60-kDa protein. Degradation bands (below the 60-kDa) were also visualized because PgmA was highly sensitive to intrinsic proteases of this bacterium [13].
Figure 5.
SDS-PAGE and CBB staining using purified FimA fimbriae.
Purified FimA fimbriae were denatured in an SDS-containing buffer with 2-mercaptoethanol by heating at 60 to 100°C for 10 min, then subjected to SDS-PAGE and CBB staining. Samples were as follows: purified from P. gingivalis ATCC 33277 Δmfa1 (native 33277 FimA fimbriae, lane 1), P. gingivalis ATCC 33277 Δmfa1 Δfim cluster with fimA of ATCC 33277 (I) (lane 2), TDC60 (II) (lane 3), 6/26 (III) (lane 4), W83 (IV) (lane 5), HG564 (IV) (lane 6), and HNA99 (V) (lane 7) introduced. Note that CBB staining did not visualize a ladder band as seen in immunoblot analysis in Fig. 2.
Figure 6.
ELISA using absorbed antisera and whole-cell sonicates as antigen.
Whole-cell sonicates were coated on ELISA plates as antigens. Antisera from mice immunized with each pure genotype fimbriae were used after absorption with the fimbria-deficient mutant 33277 Δmfa1 Δfim cluster. “Non” indicates non-immunized mouse sera. W83 rarely produces FimA protein and fimbriae. Data show mean ± SD. Asterisks indicate statistical significance compared with Non (* p<0.05, ** p<0.01). Note that scales of Y axes are adjusted in order to compare titers clearly.
Figure 7.
ELISA using absorbed antisera and purified FimA fimbriae as antigen.
Pure FimA fimbriae, derived from each fimA gene, were coated on ELISA plates as antigens. Antisera from mice immunized with each pure genotype fimbriae were used after absorption with the fimbria-deficient mutant 33277 Δmfa1 Δfim cluster. “Non” indicates non-immunized mouse sera. Data show mean ± SD. Asterisks indicate statistical significance compared with Non (* p<0.05, ** p<0.01). Note that scales of Y axes are adjusted as in Fig. 6.
Figure 8.
Immunoblot analysis using whole-cell sonicates partially denatured.
Whole-cell sonicates were denatured in an SDS-containing buffer with 2-mercaptoethanol by heating at 70°C for 10 min, and subjected to SDS-PAGE and immunoblot analysis by using antisera, 1,000-fold dilution, from mice immunized with purified FimA fimbriae. Antigen samples were as follows: P. gingivalis ATCC 33277 Δmfa1 Δfim cluster (FimA deficient, lane 1), and the wild-type strains of ATCC 33277 (lane 2), TDC60 (lane 3), 6/26 (lane 4), W83 (lane 5), HG564 (lane 6), and HNA99 (lane 7). M denotes a standard marker. W83 rarely produces FimA protein and fimbriae. Note that ladder bands are specific for FimA fimbriae whereas smear bands between 40–80 kDa are nonspecific. Arrows with dotted lines are placed in order to clearly discriminate each lane.
Figure 9.
Immunoblot analysis using whole-cell sonicates completely denatured.
Whole-cell sonicates were denatured in an SDS-containing buffer with 2-mercaptoethanol by heating at 100°C for 10 min, and subjected to SDS-PAGE and immunoblot analysis using antisera, 1,000-fold dilution, from mice immunized with purified FimA fimbriae. Antigen samples were as follows: P. gingivalis ATCC 33277 Δmfa1 Δfim cluster (FimA deficient, lane 1), and the wild-type strains of ATCC 33277 (lane 2), TDC60 (lane 3), 6/26 (lane 4), W83 (lane 5), HG564 (lane 6), and HNA99 (lane 7). M denotes standard marker. Arrowheads show distinguishable bands corresponding to FimA monomers. Note that W83 rarely produces FimA protein and fimbriae.
Figure 10.
TNF-α induction in mouse macrophage-like J774-1 cells.
Purified FimA fimbriae at 1 µg/ml and LPS at 10 EU/ml (corresponding to 2.6 ng/ml) were incubated with J774-1 cells for 3 to 24 hours. TNF-α in the medium was measured by ELISA. Medium denotes no addition; Native 33277 denotes purified FimA fimbriae from 33277 Δmfa1; 33277, TDC60, 6/26, W83, HG564 and HNA99 denote that pure FimA fimbriae, derived from the corresponding fimA in P. gingivalis ATCC 33277 Δmfa1 Δfim cluster, were used stimulants; LPS was E. coli LPS. Data show mean ± SD from two experiments with duplicate. Asterisks indicate statistical significance compared with Medium for each time (* p<0.05, ** p<0.01).
Table 1.
Summary of serological analyses.