Table 1.
The clinical characteristics of the ALI culture donors. All had a clinical, symptom based diagnosis of normal or asthma.
Figure 1.
Constitutive (16 h) and IgE-dependent (30 min) HLMC histamine secretion in monoculture and in the presence of BEAS-2B cells, either in contact or on either side of a Transwell membrane (BEAS-2B on the underside of the membrane, HLMC on the upperside of the membrane).
Mean ± SEM ng/106 cells (N = 3 HLMC donors).
Figure 2.
Constitutive (16 h) and IgE-dependent (30 min) HLMC histamine secretion in monoculture or in the presence of BEAS-2B cells separated by a Transwell membrane (HLMC in the bottom chamber, BEAS-2B in the top chamber).
Retained cell histamine content prior to activation is also shown. n = 7 HLMC donors. Mean ± SEM ng/106 cells.
Figure 3.
The effects of HLMC co-culture with healthy and asthmatic ALI AEC on constitutive (16 h) and IgE-dependent (30 min) HLMC histamine secretion compared to HLMC cultured alone, n = 6 HLMC donors.
Mean ± SEM ng/106 cells. (p<0.05*, p<0.01**, p<0.001***, ns = not significant).
Figure 4.
HLMC histamine release in different culture media.
E – primary AEC monolayer supernatant; E-SPE – SPE-processed (lipid-deplete) AEC supernatant; BEGM-SPE – SPE-processed BEGM media; BEGM – unprocessed media. Mean ± SEM of 4 experiments using 2 AEC donors and 2 HLMC donors. HLMCs cultured in AEC supernatant showed suppressed histamine release (p<0.05) whereas there is no significant suppression in lipid-deplete supernatant.
Figure 5.
The effects of AH6809, epithelial wounding and pertussis toxin on epithelial-dependent inhibition of HLMC histamine secretion n = 4 HLMC donors, mean ± SEM ng/106 cells.
(p<0.05*, p<0.01**, P<0.001***, ns = not significant).
Figure 6.
The effects of lipoxin A4, resolvin D1 and resolvin D2 on HLMC IgE-dependent degranulation.
Mean ± SEM from 7 HLMC donors. p<0.001 for all mediators (repeated measures ANOVA).
Figure 7.
LC-MS calibration curve for triplicate injections of resolvin D1 standard with a linear regression best fit line.
Integrated peak area refers to the 375.2 → 141.2 ion pair.