Figure 1.
Symptom development in tobacco leaves infected with M-CMV.
Virus-infected and mock-inoculated leaves were collected and photographed at 6 dpi (vein clearing, A), 9 dpi (mosaic, B), 11 dpi (severe chlorosis, C), 13 dpi (partial recovery, D), 16 dpi (complete recovery, E) and 20 dpi (secondary mosaic, F).
Table 1.
Summary for BLAST results of unigenes.
Table 2.
Top 20 enriched KEGG pathways.
Figure 2.
KEGG annotation of new transcripts.
The new transcripts were distributed in 60 KEGG functional classes (Release 58.1, June 1, 2011), and only the classes with new transcripts number larger than 50 were shown in the figure.
Figure 3.
Differentially-expressed genes (DEGs) in different symptom stages.
Table 3.
Important KEGG pathways influenced by M-CMV infection.
Figure 4.
Relationship of DEGs in different symptom stages.
A, relationship between DEGs at 6, 9 and 11 dpi; B, relationship between DEGs at 13 and 16 dpi; C, relationship between DEGs at 16 and 20 dpi; D, relationship between DEGs at 6 and 20 dpi.
Figure 5.
Quantitative RT-PCR (qRT-PCR) validation of the relative expression levels of transcripts selected from the DGE analysis.
Expression profiles of selected genes as determined by qRT-PCR (Red) and DGE (Blue). The signal intensity of each transcript was normalized using EF1α. The y-axis shows the normalized expression level of the transcript. The x-axis indicates the number of days post infection (dpi). Error bars represent the standard deviations of qRT-PCR signals (n = 3).
Table 4.
Primer sets and PCR efficiency of unigenes selected in qRT-PCR.