Table 1.
Goats infected with B. pseudomallei and status at euthanasia.
Figure 1.
Temperatures from selected goats.
Peaks in temperature are seen on days 1 and 2 and between days 6 and 9. Based on the temporospatial distribution of gross lesions, these peaks are likely associated with bacteremic dissemination events even though bacteremia was not detected with blood culture. BpG15 (square), BpG21 (circle), BpG22 (triangle), BpG27 (diamond).
Figure 2.
Granulocyte counts from selected goats.
During the first half of the study, the granulocyte count appears to mirror the temperature trends seen in Figure 1. BpG15 (square), BpG21 (circle), BpG22 (triangle), BpG27 (diamond), RR High (dash-dot)/Low (dot-dot): reference range limits of normal caprine granulocyte count.
Figure 3.
Thoracic and extirpated lung radiographs of goats infected with an intratracheal aerosol of
A) Goat 15, day 7, left lateral thorax with a mild bronchointerstitial pattern restricted to the caudal lung lobes. B) Goat 16, day 7, left lateral thorax with moderate to severe bronchointerstitial infiltrates with numerous ill-defined variably sized nodules in all lung lobes with the caudal lung lobes more affected. C) Goat 22, day 21, right lateral thorax with small nodules seen in all lung lobes and mild-moderate bronchointerstitial infiltrates diffusely. D) Goat 22, day 21, extirpated lungs, numerous nodules in all lung lobes, which are much more distinct than in the in vivo radiographs.
Figure 4.
Gross and histologic lesions of caprine melioidosis.
A) Lung, multiple discrete subpleural targetoid pyogranulomas with tan purulent centers and consolidated hyperemic rims; B) a splenic subcapsular pyogranuloma bulging over the splenic surface with regional capsulitis and injected vessels; C) bronchiolar epithelium showing apical surface deciliation, segmental necrosis of the pseudostratified ciliated epithelium, neutrophil transcytosis, and luminal aggregates of neutrophils suspended in inflammatory edema; D) pulmonary septal thickening secondary to neutrophil infiltration; E) sever necrosuppurative/ulcerative tracheitis with neutrophil transcytosis and a mucosal abscess (inset); F) linear renal pyogranuloma extending from the cortex down into the medulla obliterating large areas of renal parenchyma. 4C–4F hematoxylin and eosin staining.
Table 2.
Summary of organ burdens.
Figure 5.
Caprine melioidosis vasculitides in multiple organs.
A) pulmonary pyogranuloma encroaching on large interlobular vein, resulting in a segmental leukocytoclastic vasculitis – unaffected endothelium is present on the contralateral side of the vessel; B) pulmonary vasculitis in a medium-sized artery demonstrating a clear separation of the tunica intima from the tunica media by neutrophils and macrophages with segmental intimal proliferation; C) splenic capsular vasculitis with perivascular neutrophilic infiltrate; D) vasocentric renal pyogranuloma showing neutrophil infiltration into the subintima and adventitia with endothelial proliferation and fibrinoid necrosis of a small renal vessel E) focal vasculitis and early pyogranuloma formation at the corticomedullary junction of the adrenal gland; F) leukocytoclastic arteritis in the testicle with an adjacent area of testicular degeneration showing diminished stratification of spermatogenic cells and interstitial suppurative orchitis. 5A–5F, hematoxylin and eosin staining.