Table 1.
Genotype of selected M. tuberculosis strains.
Figure 1.
Growth of M. tuberculosis strains in liquid 7H9 broth.
Fig. 1 (A) Growth of individual M. tuberculosis strains from different lineages in liquid 7H9 broth. Data represent the mean and range (n = 2) values. Fig. 1 (B) Summarized growth of M. tuberculosis strain lineages. Fig. 1 (C) Comparison of Cmax (maximum point on the growth curve) for different strain lineages. Lineage 4 strains showed higher Cmax than lineage 2**(p = 0.01) and lineage 3*** strains (p = 0.001). H37Rv showed significantly higher Cmax than all clinical strains# (p = 0.001) Fig. 1 (D) Comparison of Tmax (time to reach to Cmax) for different strain lineages. H37Rv reached Tmax significantly faster than lineage 3 strains* (p = 0.05).
Table 2.
Strain wise comparison of M. tuberculosis growth in axenic media based on Cmax # and Tmax # # values.
Figure 2.
Strain uptake by monocyte derived macrophages.
Fig. 2 (A) Uptake of M. tuberculosis strains by MDM (4 h CFU calculated as a percentage of initial inoculum). Fig. 2 (B) Summarized uptake of M. tuberculosis strain lineages by MDM. H37Rv showed significantly lower uptake by the than clinical strains# (p<0.001). Data represent the mean ± SD (n = 7).
Figure 3.
Growth index reflecting the intracellular growth of M. tuberculosis strains in MDM.
Fig. (3A) Growth index of M. tuberculosis strains (calculated by the CFU at each time point divided by the CFU at initial time point). Fig. (3B) Summarized growth index of M. tuberculosis strain lineages. Data represent the mean ± SD (n = 7). Statistical significance is denoted as *p<0.05, **p<0.01, ***p<0.001.
Table 3.
Doubling time+ for M. tuberculosis strains from different lineages in human monocyte derived macrophages.
Figure 4.
Cytokines detected in culture-supernatant (at 48 h) from MDM infected by different strains of M. tuberculosis.
Fig. 4 (A) IL-12p40 (n = 6 donors). H37Rv and lineage 4 strains induced significantly higher level of IL-12p40 as compared to lineage 3# and lineage 2# lineages (p<0.001 for both comparisons) Fig. 4 (B) TNF (n = 8 donors). Lineage 3 strains induced significantly higher levels of TNF as compared to lineage 2# (p = 0.001), lineage 4* (p<0.05) and H37Rv** (p = 0.01). H37Rv and lineage 4 strains induced higher level of TNF than lineage 2 strain (lineage 4 vs lineage 2 p = 0.001, H37Rv vs lineage 2 p = 0.01). Data represent the median and range.