Figure 1.
a) Microscope image of the microfabricated NMR insert. b) Profile of the electroplated on-chip capacitor. c) Schematic of the magic angle coil spinning (MACS) experimental set-up.
Figure 2.
Successful tuning of the microfabricated NMR insert at 500 MHz.
Figure 3.
Sensitivity of the microfabricated NMR insert.
1H spectrum at 700 MHz of a mixture of 99.9% D2O and 0.1% H2O.
Figure 4.
500 MHz 1H NMR spectrum of 330 nl water spun at 110 Hz.
The isotropic central peak is split in sidebands spaced at 110 Hz, i.e., 0.22 ppm at 500 MHz. The inset shows the central water peak which has a non-Gaussian shape due to the field inhomogeneities (see Discussion section).
Figure 5.
1H NMR spectra of Drosophila pupae.
The spectra was acquired with a total of three pupae in a 400 µm-microcoil coupled with a standard 7-mm MAS probe, spinning at 500 Hz for the single-pulse experiment, and at 371 Hz for the PASS experiment. The total experimental time for the PASS experiment is about 1 hour. Peak assignment (1) Lipid –CH3 (2) fatty acid –(CH2)n (3) lactate CH3 (4) Lipid –CH2-CH2-CO– (5) Lipid–CH = CH-CH2-CH2– (6) Lipid –CH2-CH2-CO–.
Figure 6.
Linewidth narrowing of solid-state adamantane spectrum for higher rotational speeds.
NMR spectra at 500 MHz for two different spinning speeds: 0.9 ppm at 10 kHz vs. 1.5 ppm at 5 kHz.