Figure 1.
Sixteen week old BALB/c mice were ovariectomized (OVX) and maintained in the vivarium for 57 days in order to attain skeletal hemostasis. Both intact (I) and OVX mice were then irradiated with 16Gy delivered to the caudal skeleton or maintained as controls. Groups of animals were euthanized at 3, 8, and 30 days post irradiation in order to perform histological evaluations of the distal femur; microCT measurements were conducted at the 30 day time point only.
Figure 2.
Schematic representation of bone and marrow.
A. region of interest in sagittal view of a normal control mouse. B. Sagittal section of bone marrow from an intact mouse 8 days post-irradiation. Note the areas of congestion and sinusoidal dilation characterized by increased density of red blood cell within the expanded vascular spaces; also present are areas of edema characterized by increased volume of pale pink fluid in the interstitial spaces. C. Sagittal sections of bone marrow 30 days after irradiation in intact (C1) and OVX (C2) mice. Note the more intensely purple areas indicative of higher hematopoietic activity in C1 compared with C2, as well as expanded adipose in C2 compared with C1.
Figure 3.
Percent of the marrow space (mean ±SEM) occupied by hematopoietic precursors in intact control (I−R) and irradiated (I+R) mice estimated by visual inspection by a board certified veterinary clinical pathologist (LS) employing standard protocols.
Radiation caused marked hypocellularity of the hematopoietic components of the marrow by Day 3, with significant increases but still very low hematopoietic activity on Day 8 and marked but still incomplete recovery of hematopoietic activity with normal precursor maturation at Day 30. There were no changes over time in non-irradiated animals. Two-way ANOVA with Tukey's post-tests: * Irradiated mice had significantly lower cellularity than control mice of the same day, p<0.0001, † Cellularity of irradiated mice increased significantly from Day 3 through Day 30, p: 0.0292 – <0.0001.
Table 1.
Megakaryocyte number and myeloid: erythroid ratio in intact control (I−R) and irradiated (I+R) mice estimated by a board certified veterinary clinical pathologist (LS) according to standard protocols.
Table 2.
Semi-quantitative assessment of vascular abnormalities in intact control (I−R) and irradiated (I+R) mice estimated by a board certified veterinary clinical pathologist (LS) according to standard protocols.
Table 3.
Comparison of group least squares means (± SEM) of hematopoietic cellularity, adiposity, and bone volume in intact (I) and ovariectomized (OVX) mice without radiation (−R) and with radiation (+R) 30 days after radiation treatment.
Table 4.
Megakaryocyte number and myeloid: erythroid ratio in intact (I) and ovariectomized (OVX) mice receiving either 0 (−R) or 16 Gy radiation (+R) 30 days previously.
Table 5.
Congestion and fibrosis in intact and ovariectomized (OVX) mice receiving either 0 or 16 Gy radiation 30 days previously.
Figure 4.
Ovariectomy influences the relationships between bone volume (BV/TV%), adipogenesis (% marrow fat), and hematopoiesis (% cellularity) at Day 30 with or without 16 Gy radiation to the hind limb in mice.
Data of every two measurements are represented as least squares mean values for the group. Panel A: OVX results in a 10 fold reduction in the rate of bone volume loss per unit increase in marrow fat after radiation treatment compared with intact mice. Panel B: OVX blunts the rate of bone volume loss relative to reduced hematopoietic cellularity after radiation compared with intact mice by a factor of 6. Panel C: OVX halves the reduction in hematopoietic cellularity compared with increased marrow fat after radiation treatment compared with intact mice.
Figure 5.
3-D illustration of interrelationships among the three tissue components of bone and marrow: hemopoietic component measured by cellularity, stromal damage component measured by marrow fat or adipose content, and osseous component measured by the cancellous bone BV/TV%.
Cumulative increases in marrow fat after irradiation, in the absence of ovarian function (10 fold) was not reflected by equivalent losses of either cancellous bone or hematopoietic cellularity. The proportionality of changes in these tissue components were maintained among irradiated intact mice.