Figure 1.
Summary of the experimental workflow.
A. Emission spectrum of the UVB lamps used to irradiate the cultured cells of P. angustum B. Bacterial cells were collected at the beginning of log phase (OD = 0.1) and cultured either under UVB radiation or in the dark C. After 1.75 h of incubation, quantitative proteomics was performed by using either gel-free (Post-digest ICPL labeling) or gel-based (2D-DIGE) approaches. Quantitative data for ICPL were obtained from the MS spectrum (L: Light label: H: Heavy label).
Table 1.
List of the quantified proteins in four biological replicates using the post-digest ICPL labeling methodology.
Figure 2.
Venn diagram showing the non-redundant proteins quantified in four biological replicates.
Figure 3.
Comparison of the different steps of protein identification and quantification between the two experimental workflows.
Figure 4.
A representative standard 2D gel showing protein spots that were down-regulated (A) and up-regulated (B).
Proteins of interest are circled in green, and excised spots are circled in blue or red according their relative spot volumes (D = down-regulated by UVB; U = up-regulated by UVB). Green circles correspond to the up- or down-regulated proteins not excised for MS analysis.
Table 2.
Identification of the two-dimensionally separated protein spots of interest with the average fold change and associated p-values obtained from seven replicates.
Table 3.
UVB protein biomarkers of P. angustum quantified by both ICPL and 2D-DIGE.
Figure 5.
Detection of the RecA protein (≈40 kDa) by quantitative fluorescent western blot analysis of both UVB-treated cells and dark control cultures.
Four different biological replicates are shown for each condition.
Figure 6.
Diagram depicting the cellular pathways influenced by UVB radiation in P. angustum.
The up- and down-regulated proteins are represented in red and blue, respectively.