Table 1.
Gene-specific primers and annealing temperatures used for qRT-PCR.
Figure 1.
The growth curves of treatments and control during the course of cultivation.
Figure 2.
Microscopic images (400×) of H. pluvialis cells culture samples day 3 and 18 after treatment with JA.
(A), (B), (C) represent the control, JA25 sample and JA50 sample after 3 days of treatment, respectively; (D), (E), (F) represent the control, JA25 sample and JA50 sample after 18 days of treatment, respectively; arrow 1 and arrow 2 show the algae cells were whitened or autolysed in the JA50 samples.
Figure 3.
Astaxanthin accumulations of control, JA25 sample and JA50 sample during cultivation.
OD490 represents relative astaxanthin content in alga culture solution.
Figure 4.
Changes of three photosynthesis fluorescence parameters of control, JA25 sample and JA50 sample on day 0, 3, 6, 9, 12, 15, 18 of incubation.
(A), (B), (C) represent changs of Fv/Fm, Y (II) and NPQ, respectively.
Figure 5.
The effects of JA on the transcript levels expression kinetics of eight carotenogenic genes in H. pluvialis during incubation.
(A), (B), (C), (D), (E), (F), (G) and (H) represent transcript levels expression kinetics of ipi-1, ipi-2, psy, pds, crtR-B, lyc, bkt2 and crtO respectively.