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Figure 1.

Time–kill curve analysis of one hVISA (R3003, VAN MIC = 2, NAF MIC = 256 µg/mL) isolate.

All antimicrobials are at a concentration of 0.5x MIC. This isolate shows synergy between vancomycin and nafcillin. Data are presented as a graph of the mean bacteria remaining vs. time with error bars at the sampling points representing 1 standard deviation from the mean. GC = Growth Control, NAF = Nafcillin, VAN = Vancomycin.

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Figure 2.

Activity of vancomycin and nafcillin alone and in combination in the PK/PD model against hVISA strains where the concentration of nafcillin was below the MIC of the respective organism for 100% of the dosing interval.

Graphs shown are of isolate R5253 (Nafcillin MIC = 16 µg/mL) (A), R1915 (Nafcillin MIC = 64 µg/mL) (B), R2729 (Nafcillin MIC = 128 µg/mL) (C), and R3003 (Nafcillin MIC = 256 µg/mL) (D). Data are presented as a graph of the mean bacteria remaining vs. time with error bars at the sampling points representing 1 standard deviation from the mean. GC = Growth Control, NAF = Nafcillin, VAN = Vancomycin.

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Figure 2 Expand

Figure 3.

Activity of vancomycin and nafcillin alone and in combination in the PK/PD model against the hVISA strain with a nafcillin MIC of 4 µg/mL (R1629).

Data are presented as a graph of the mean bacteria remaining vs. time with error bars at the sampling points representing 1 standard deviation from the mean. GC = Growth Control, NAF = Nafcillin, VAN = Vancomycin.

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Figure 3 Expand

Figure 4.

Activity of vancomycin and nafcillin alone and in combination in the PK/PD model against one MSSA isolate (SNL9) (A) and one MRSA isolate (SNL96) (B).

Data are presented as a graph of the mean bacteria remaining vs. time with error bars at the sampling points representing 1 standard deviation from the mean. GC = Growth Control, NAF = Nafcillin, VAN = Vancomycin.

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Figure 4 Expand

Table 1.

Area under the bacterial kill curve values from the PK/PD models.

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Table 1 Expand